{"doi":"10.1242/jcs.239616","title":"Cep44 functions in centrosome cohesion by stabilizing rootletin","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>The centrosome linker serves to hold the duplicated centrosomes together until they separate in late G2/early mitosis. Precisely how the linker is assembled remains an open question. In this study, we identify Cep44 as a novel component of the linker in human cells. Cep44 localizes to the proximal end of centrioles, including mother and daughter centrioles, and its ablation leads to loss of centrosome cohesion. Cep44 does not impinge on the stability of C-Nap1 (also known as CEP250), LRRC45 or Cep215 (also known as CDK5RAP2), and vice versa, and these proteins are independently recruited to the centrosome. Rather, Cep44 associates with rootletin and regulates its stability and localization to the centrosome. Our findings reveal a role of the previously uncharacterized protein Cep44 for centrosome cohesion and linker assembly.</jats:p>","journal":"Journal of Cell Science","year":2020,"id":47230,"datarank":0.6767258516494927,"base_score":3.044522437723423,"endowment":3.044522437723423,"self_citation_contribution":0.4566783656585135,"citation_network_contribution":0.2200474859909792,"self_endowment_contribution":0.4566783656585135,"citer_contribution":0.2200474859909792,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":20,"citers_with_citation_signal":13,"citers_with_endowment":13,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":217887,"name":"Sunny Y.-P. Shih","orcid":null,"position":1,"is_corresponding":false},{"id":217888,"name":"Xintong Xiao","orcid":null,"position":2,"is_corresponding":false},{"id":217889,"name":"Julia White","orcid":null,"position":3,"is_corresponding":false},{"id":217890,"name":"William Y. Tsang","orcid":"0000-0002-1584-616X","position":4,"is_corresponding":false},{"id":217886,"name":"Delowar Hossain","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Cep44 functions in centrosome cohesion by stabilizing rootletin","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>The centrosome linker serves to hold the duplicated centrosomes together until they separate in late G2/early mitosis. Precisely how the linker is assembled remains an open question. In this study, we identify Cep44 as a novel component of the linker in human cells. Cep44 localizes to the proximal end of centrioles, including mother and daughter centrioles, and its ablation leads to loss of centrosome cohesion. Cep44 does not impinge on the stability of C-Nap1 (also known as CEP250), LRRC45 or Cep215 (also known as CDK5RAP2), and vice versa, and these proteins are independently recruited to the centrosome. Rather, Cep44 associates with rootletin and regulates its stability and localization to the centrosome. Our findings reveal a role of the previously uncharacterized protein Cep44 for centrosome cohesion and linker assembly.</jats:p>","is_dataset_classified":null,"base_score":3.044522437723423,"endowment":3.044522437723423,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31974111","pmcid":"PMC7044459","openalex_id":"https://openalex.org/W3001414175","authors":[],"funders":[{"funder_name":"Natural Sciences and Engineering Research Council of Canada","grant_id":"RGPIN-2016-04002","title":null},{"funder_name":"Cancer Research Society","grant_id":"2018-23026","title":null},{"funder_name":"Natural Sciences and Engineering Research Council of Canada","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Fonds de recherche Santé","grant_id":"","title":null}],"total_grants":4,"fwci":0.9851,"citation_percentile":0.70716133,"influential_citations":1,"citation_trend":[{"year":2021,"count":4},{"year":2022,"count":4},{"year":2023,"count":1},{"year":2024,"count":5},{"year":2025,"count":4},{"year":2026,"count":2}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://journals.biologists.com/jcs/article-pdf/133/4/jcs239616/1990879/jcs239616.pdf","host_type":"journal"},{"url":"https://doi.org/10.1242/jcs.239616","host_type":"HYBRID"},{"url":"https://journals.biologists.com/jcs/article-pdf/133/4/jcs239616/1990879/jcs239616.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1242/jcs.239616","host_type":"publisher"},{"url":"https://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.239616/3513255/jcs239616.pdf","host_type":"publisher"},{"url":"http://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.239616/2062370/jcs_239616v1.pdf","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/31974111","host_type":"repository"},{"url":"http://jcs.biologists.org/cgi/content/short/133/4/jcs239616","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7044459","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC7044459","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1242/jcs.239616","host_type":""},{"url":"https://dx.doi.org/10.1242/jcs.239616","host_type":""}],"fields_of_study":["Microtubule and mitosis dynamics","Cellular transport and secretion","Cellular Mechanics and Interactions","Medicine","Biology","0301 basic medicine","0303 health sciences","03 medical and health sciences","Autoantigens","Cell Cycle Proteins","Centrioles","Centrosome","Cytoskeletal Proteins","Humans","Mitosis","Nerve Tissue Proteins"],"mesh_terms":["Autoantigens","Centrioles","Cytoskeletal Proteins","Humans","Mitosis","Nerve Tissue Proteins","Centrosome","Cell Cycle Proteins"],"keywords":["Biology","Centrosome","Cohesion (chemistry)","Cell biology","Evolutionary biology","Genetics","Cell cycle","Cell","Splitting","Cohesion","Rootletin","Cep44","Cytoskeletal Proteins","Short Report","Humans","Mitosis","Cell Cycle Proteins","Nerve Tissue Proteins","Autoantigens","Centrioles"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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