{"doi":"10.1242/jcs.237990","title":"Distinct roles of two eIF4E isoforms in the germline of <i>Caenorhabditis elegans</i>","abstract":"ABSTRACT Germ cells use both positive and negative mRNA translational control to regulate gene expression that drives their differentiation into gametes. mRNA translational control is mediated by RNA-binding proteins, miRNAs and translation initiation factors. We have uncovered the discrete roles of two translation initiation factor eIF4E isoforms (IFE-1, IFE-3) that bind 7-methylguanosine (m7G) mRNA caps during Caenorhabditis elegans germline development. IFE-3 plays important roles in germline sex determination (GSD), where it promotes oocyte cell fate and is dispensable for spermatogenesis. IFE-3 is expressed throughout the germline and localizes to germ granules, but is distinct from IFE-1 and PGL-1, and facilitates oocyte growth and viability. This contrasts with the robust expression in spermatocytes of IFE-1, the isoform that resides within P granules in spermatocytes and oocytes, and promotes late spermatogenesis. Each eIF4E is localized by its cognate eIF4E-binding protein (IFE-1:PGL-1 and IFE-3:IFET-1). IFE-3 and IFET-1 regulate translation of several GSD mRNAs, but not those under control of IFE-1. Distinct mutant phenotypes, in vivo localization and differential mRNA translation suggest independent dormant and active periods for each eIF4E isoform in the germline.","journal":"Journal of Cell Science","year":2020,"id":96337,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9545,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":477550,"name":"Jacob Subash","orcid":"0000-0002-0326-8497","position":1,"is_corresponding":false},{"id":478274,"name":"Hamilton J. Stoffel","orcid":null,"position":2,"is_corresponding":false},{"id":477551,"name":"Melissa A. Henderson","orcid":"0000-0002-6966-7749","position":3,"is_corresponding":false},{"id":478275,"name":"Jenna L. Hoffman","orcid":null,"position":4,"is_corresponding":false},{"id":478276,"name":"David S. Buckner","orcid":null,"position":5,"is_corresponding":false},{"id":478277,"name":"Madhu S. Sengupta","orcid":null,"position":6,"is_corresponding":false},{"id":477552,"name":"Peter R. Boag","orcid":"0000-0002-0889-0859","position":7,"is_corresponding":false},{"id":453210,"name":"Myon‐Hee Lee","orcid":"0000-0001-9020-2391","position":8,"is_corresponding":false},{"id":477553,"name":"Brett D. Keiper","orcid":"0000-0002-8621-9825","position":9,"is_corresponding":false},{"id":478273,"name":"Hayden P. Huggins","orcid":null,"position":0,"is_corresponding":true}],"reference_count":79,"raw_metadata":null,"created_at":"2026-07-18T22:34:34.781847Z","pmid":"32079657","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}