{"doi":"10.1242/jcs.209080","title":"Citron kinase-dependent F-actin maintenance at midbody secondary ingression sites mediates abscission","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>Abscission is the final step of cytokinesis whereby the intercellular bridge (ICB) linking the two daughter cells is cut. The ICB contains a structure called the midbody, required for the recruitment and organization of the abscission machinery. Final midbody severing is mediated by formation of secondary midbody ingression sites, where the ESCRT III component CHMP4B is recruited to mediate membrane fusion. It is presently unknown how cytoskeletal elements cooperate with CHMP4B to mediate abscission. Here, we show that F-actin is associated with midbody secondary sites and is necessary for abscission. F-actin localization at secondary sites depends on the activity of RhoA and on the abscission regulator citron kinase (CITK). CITK depletion accelerates loss of F-actin proteins at the midbody and subsequent cytokinesis defects are reversed by restoring actin polymerization. Conversely, midbody hyperstabilization produced by overexpression of CITK and ANLN is reversed by actin depolymerization. CITK is required for localization of F-actin and ANLN at the abscission sites, as well as for CHMP4B recruitment. These results indicate that control of actin dynamics downstream of CITK prepares the abscission site for the final cut.</jats:p>","journal":"Journal of Cell Science","year":2018,"id":42111,"datarank":1.3684899472337138,"base_score":3.6375861597263857,"endowment":3.6375861597263857,"self_citation_contribution":0.5456379239589579,"citation_network_contribution":0.8228520232747558,"self_endowment_contribution":0.5456379239589579,"citer_contribution":0.8228520232747558,"corpus_percentile":null,"corpus_rank":null,"citation_count":37,"citer_count":30,"citers_with_citation_signal":27,"citers_with_endowment":27,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":48.75,"fair_percentile":44.94283201407212,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":201990,"name":"Francesca Macaluso","orcid":null,"position":1,"is_corresponding":false},{"id":201991,"name":"Francesco Sgrò","orcid":"0000-0002-2062-4908","position":2,"is_corresponding":false},{"id":201992,"name":"Gaia E. Berto","orcid":"0000-0001-5963-6298","position":3,"is_corresponding":false},{"id":201993,"name":"Federico T. Bianchi","orcid":null,"position":4,"is_corresponding":false},{"id":201994,"name":"Alessandra A. Chiotto","orcid":"0000-0002-9692-1714","position":5,"is_corresponding":false},{"id":201995,"name":"Gianmarco Pallavicini","orcid":null,"position":6,"is_corresponding":false},{"id":201996,"name":"Ferdinando Di Cunto","orcid":"0000-0001-9367-6357","position":7,"is_corresponding":false},{"id":201997,"name":"Marta Gai","orcid":"0000-0002-9819-1743","position":8,"is_corresponding":false},{"id":201989,"name":"Alessandro Dema","orcid":"0000-0003-0976-9396","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.6375861597263857,"endowment":3.6375861597263857,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29588396","pmcid":null,"openalex_id":"https://openalex.org/W2794745715","authors":[],"funders":[{"funder_name":"Associazione Italiana per la Ricerca sul Cancro","grant_id":"IG17527","title":null},{"funder_name":"Fondazione 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Ricerche","grant_id":"EPIGEN","title":null}],"total_grants":4,"fwci":2.5052,"citation_percentile":0.90121455,"influential_citations":0,"citation_trend":[{"year":2018,"count":1},{"year":2019,"count":9},{"year":2020,"count":9},{"year":2021,"count":8},{"year":2022,"count":3},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"bronze","license":"http://www.biologists.com/user-licence-1-1/","oa_locations":[{"url":"https://jcs.biologists.org/content/joces/131/8/jcs209080.full.pdf","host_type":"journal"},{"url":"https://jcs.biologists.org/content/joces/131/8/jcs209080.full.pdf","host_type":"BRONZE"},{"url":"https://jcs.biologists.org/content/joces/131/8/jcs209080.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1242/jcs.209080","host_type":"publisher"},{"url":"https://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.209080/3505195/jcs209080.pdf","host_type":"publisher"},{"url":"http://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.209080/2058396/jcs_209080v1.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/jcs.209080","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29588396","host_type":"repository"},{"url":"http://hdl.handle.net/2318/1674138","host_type":"repository"},{"url":"http://jcs.biologists.org/cgi/content/short/131/8/jcs209080","host_type":"repository"}],"fields_of_study":["Signaling 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