{"doi":"10.1242/jcs.201905","title":"Essential and selective role of SNX12 in transport of endocytic and retrograde cargo","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>The endosomal protein-sorting machineries play vital roles in diverse physiologically important cellular processes. Much of the core membrane-sorting apparatus is conserved in evolution, such as retromer, which is involved in the recycling of a diverse set of cargoes via the retrograde trafficking route. Here, in an RNAi-based loss-of-function study, we identified that suppression of SNX12 leads to a severe blockage in CIM6PR (also known as IGF2R) transport and alters the morphology of the endocytic compartments. We demonstrate that SNX12 is involved in the early phase of CIM6PR transport, and mediates receptor recycling upstream of the other well-established SNX components of retromer. Ultra-structural analysis revealed that SNX12 resides on tubulo-vesicular structures, despite it lacking a BAR domain. Furthermore, we illustrate that SNX12 plays a key role in intraluminal vesicle formation and in the maturation of a subpopulation of early endosomes into late endosomes, thereby regulating selective endocytic transport of cargo for degradation. This study therefore provides evidence for the existence of early endosomal subpopulations that have differential roles in the sorting of the cargoes along endocytic degradative pathways.</jats:p>","journal":"Journal of Cell Science","year":2017,"id":24044,"datarank":0.9049477841714185,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.434623651782046,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.434623651782046,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":20,"citers_with_citation_signal":17,"citers_with_endowment":17,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":145509,"name":"Jini Sugatha","orcid":null,"position":1,"is_corresponding":false},{"id":145510,"name":"Sameena Parveen","orcid":null,"position":2,"is_corresponding":false},{"id":145511,"name":"Sandra Lacas-Gervais","orcid":null,"position":3,"is_corresponding":false},{"id":145512,"name":"Prateek Raj","orcid":null,"position":4,"is_corresponding":false},{"id":145513,"name":"Jérôme Gilleron","orcid":null,"position":5,"is_corresponding":false},{"id":145514,"name":"Sunando Datta","orcid":"0000-0002-1417-0276","position":6,"is_corresponding":false},{"id":145508,"name":"Amulya Priya","orcid":"0000-0001-8673-5371","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28705836","pmcid":null,"openalex_id":"https://openalex.org/W2735363791","authors":[],"funders":[{"funder_name":"Department of Science and Technology, Ministry of Science and Technology","grant_id":"IGSTC/MPG/PG(SD)/2011 dt 19-12-2011","title":null}],"total_grants":1,"fwci":1.2643,"citation_percentile":0.77694802,"influential_citations":3,"citation_trend":[{"year":2017,"count":1},{"year":2018,"count":2},{"year":2019,"count":1},{"year":2020,"count":6},{"year":2021,"count":3},{"year":2022,"count":3},{"year":2023,"count":2},{"year":2024,"count":2},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"bronze","license":"http://www.biologists.com/user-licence-1-1/","oa_locations":[{"url":"https://jcs.biologists.org/content/joces/130/16/2707.full.pdf","host_type":"journal"},{"url":"https://jcs.biologists.org/content/joces/130/16/2707.full.pdf","host_type":"HYBRID"},{"url":"https://jcs.biologists.org/content/joces/130/16/2707.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1242/jcs.201905","host_type":"publisher"},{"url":"https://journals.biologists.com/jcs/article-pdf/130/16/2707/3499487/jcs201905.pdf","host_type":"publisher"},{"url":"http://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.201905/2057810/jcs_201905v1.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/jcs.201905","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28705836","host_type":"repository"},{"url":"https://hal.science/hal-02108393","host_type":"repository"},{"url":"http://jcs.biologists.org/cgi/content/short/130/16/2707","host_type":"repository"}],"fields_of_study":["Cellular transport and secretion","Lipid Membrane Structure and Behavior","Retinal Development and Disorders","Biology","Medicine","Biological Transport","Cholesterol, LDL","Endocytosis","Endosomes","Epidermal Growth Factor","ErbB Receptors","HEK293 Cells","HeLa Cells","Humans","Metabolic Networks and Pathways","Protein Transport","Proteolysis","RNA Stability","Sorting Nexins","Transport Vesicles"],"mesh_terms":["Biological Transport","Endocytosis","Epidermal Growth Factor","HeLa Cells","Humans","Cholesterol, LDL","Endosomes","RNA Stability","Protein Transport","Transport Vesicles","Metabolic Networks and Pathways","HEK293 Cells","Sorting Nexins","Proteolysis","ErbB Receptors","Hela Cells"],"keywords":["Endosome","Endocytic cycle","Retromer","Biology","Sorting nexin","Cell biology","Vesicular Transport Proteins","Transport protein","Protein targeting","Endocytosis","Vacuolar protein sorting","Membrane protein","Receptor","Biochemistry","Membrane","EGF","Intraluminal Vesicles","Cim6pr","Endosomal Maturation","Snx12"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-07T21:17:51.457839Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}