{"doi":"10.1242/jcs.182824","title":"Endosomal assembly and transport of heteromeric septin complexes promote septin cytoskeleton formation","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>Septins are conserved cytoskeletal structures functioning in a variety of biological processes including cytokinesis and cell polarity. A wealth of information exists on the heterooligomeric architecture of septins and their subcellular localization at distinct sites. However, the precise mechanisms of their subcellular assembly and their intracellular transport are unknown. Here, we demonstrate that endosomal transport of septins along microtubules is crucial for formation of higher-order structures in the fungus Ustilago maydis. Importantly, endosomal septin transport is dependent on each individual septin providing strong evidence that septin heteromeric complexes are assembled on endosomes. Furthermore, endosomal trafficking of all four septin mRNAs is required for endosomal localization of their translation products. Based on these results, we propose that local translation promotes the assembly of newly synthesized septins in heteromeric structures on the surface of endosomes. This is important for the long-distance transport of septins and the efficient formation of the septin cytoskeleton.</jats:p>","journal":"Journal of Cell Science","year":2016,"id":682423,"datarank":0.6435689161722588,"base_score":4.290459441148391,"endowment":4.290459441148391,"self_citation_contribution":0.6435689161722588,"citation_network_contribution":0.0,"self_endowment_contribution":0.6435689161722588,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":72,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1782836,"name":"Sebastian Baumann","orcid":null,"position":1,"is_corresponding":false},{"id":1782837,"name":"Stefanie Weidtkamp-Peters","orcid":null,"position":2,"is_corresponding":false},{"id":1532182,"name":"Michael Feldbrügge","orcid":"0000-0003-0046-983X","position":3,"is_corresponding":false},{"id":1782834,"name":"Sabrina Zander","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Endosomal assembly and transport of heteromeric septin complexes promote septin cytoskeleton formation","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>Septins are conserved cytoskeletal structures functioning in a variety of biological processes including cytokinesis and cell polarity. A wealth of information exists on the heterooligomeric architecture of septins and their subcellular localization at distinct sites. However, the precise mechanisms of their subcellular assembly and their intracellular transport are unknown. Here, we demonstrate that endosomal transport of septins along microtubules is crucial for formation of higher-order structures in the fungus Ustilago maydis. Importantly, endosomal septin transport is dependent on each individual septin providing strong evidence that septin heteromeric complexes are assembled on endosomes. Furthermore, endosomal trafficking of all four septin mRNAs is required for endosomal localization of their translation products. Based on these results, we propose that local translation promotes the assembly of newly synthesized septins in heteromeric structures on the surface of endosomes. This is important for the long-distance transport of septins and the efficient formation of the septin cytoskeleton.</jats:p>","is_dataset_classified":null,"base_score":4.290459441148391,"endowment":4.290459441148391,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27252385","pmcid":null,"openalex_id":"https://openalex.org/W2409064995","authors":[],"funders":[{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"EXC 1028","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"DFG FE448/9-1","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"CRC1208","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"unidentified","title":"unidentified"},{"funder_name":"iGRAD Molecules of Infection","grant_id":"","title":null}],"total_grants":5,"fwci":5.6117,"citation_percentile":0.96466226,"influential_citations":0,"citation_trend":[{"year":2016,"count":4},{"year":2017,"count":10},{"year":2018,"count":14},{"year":2019,"count":7},{"year":2020,"count":8},{"year":2021,"count":9},{"year":2022,"count":4},{"year":2023,"count":2},{"year":2024,"count":7},{"year":2025,"count":5},{"year":2026,"count":2}],"oa_status":"bronze","license":"http://www.biologists.com/user-licence-1-1","oa_locations":[{"url":"https://jcs.biologists.org/content/joces/129/14/2778.full.pdf","host_type":"journal"},{"url":"https://jcs.biologists.org/content/joces/129/14/2778.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1242/jcs.182824","host_type":"publisher"},{"url":"https://journals.biologists.com/jcs/article-pdf/129/14/2778/3495418/jcs182824.pdf","host_type":"publisher"},{"url":"http://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.182824/2049452/jcs_182824v1.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/jcs.182824","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27252385","host_type":"repository"},{"url":"http://jcs.biologists.org/cgi/content/short/129/14/2778","host_type":"repository"},{"url":"http://jcs.biologists.org/content/129/14/2778.full.pdf","host_type":""},{"url":"https://dx.doi.org/10.1242/jcs.182824","host_type":""}],"fields_of_study":["Fungal and yeast genetics research","Plant Pathogens and Fungal Diseases","Plant Reproductive Biology","0301 basic medicine","0303 health sciences","03 medical and health sciences","Cytoskeleton","Endosomes","Fungal Proteins","Microtubules","Models, Biological","Protein Binding","Protein Multimerization","RNA, Messenger","Septins","Subcellular Fractions","Ustilago"],"mesh_terms":["Cytoskeleton","Fungal Proteins","Microtubules","Models, Biological","Protein Binding","Endosomes","RNA, Messenger","Subcellular Fractions","Ustilago","Protein Multimerization","Septins"],"keywords":["Septin","Endosome","Biology","Cell biology","Cytoskeleton","Microtubule","Cytokinesis","ESCRT","Translation (biology)","Transport protein","Intracellular","Cell division","Cell","Biochemistry","Gene","Messenger RNA","RNA recognition motif","Early Endosomes","Mrna Transport","Endosomes","Microtubules","Models, Biological","Fungal Proteins","Ustilago","RNA, Messenger","Protein Multimerization","Septins","Protein Binding","Subcellular Fractions"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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