{"doi":"10.1242/jcs.179200","title":"Rop, the Sec1/Munc18 homolog in<i>Drosophila</i>, is required for furrow ingression and stable cell shape during cytokinesis","abstract":"<jats:p>Physically separating daughter cells during cytokinesis requires contraction of an actin-myosin ring and vesicle-mediated membrane addition at the cleavage furrow. To identify vesicle trafficking proteins that function in cytokinesis, we screened deficiencies and mutations of candidate genes by live-imaging the mitotic domains of the Drosophila embryo. In embryos homozygous for some of these deficiencies, we observed several cytokinesis phenotypes, including slow furrow ingression and increased membrane blebbing. We also found that cytokinesis required the Sec1/Munc18 homolog, Rop, which interacts with syntaxin and mediates exocytosis at the plasma membrane. In a temperature sensitive Rop mutant (RopTS), the contractile ring disassembled during furrow ingression, indicating that maintenance of the ring required vesicle addition. Furthermore, in some dividing RopTS cells, the shape of the daughter cells became unstable, causing cytokinesis failure. These results further highlight the importance of vesicle trafficking in animal cytokinesis and show that vesicle fusion influences cell shape during cytokinesis.</jats:p>","journal":"Journal of Cell Science","year":2015,"id":42094,"datarank":0.31555249370956207,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.07413680684444701,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.07413680684444701,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":3,"citers_with_citation_signal":3,"citers_with_endowment":3,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":201925,"name":"Roger Albertson","orcid":null,"position":1,"is_corresponding":false},{"id":201926,"name":"Zachary Swider","orcid":null,"position":2,"is_corresponding":false},{"id":201927,"name":"William Sullivan","orcid":null,"position":3,"is_corresponding":false},{"id":201924,"name":"Heather DeBruhl","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26631487","pmcid":"PMC4732290","openalex_id":"https://openalex.org/W2193264453","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"5 R01 GM046409","title":null},{"funder_name":"National Institutes of Health","grant_id":"5 F32 GM075670","title":null},{"funder_name":"Albion College","grant_id":"Faculty Development Grant","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"F32 GM075670","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM046409","title":null}],"total_grants":5,"fwci":0.0,"citation_percentile":0.08018082,"influential_citations":0,"citation_trend":[{"year":2020,"count":1},{"year":2021,"count":1},{"year":2022,"count":1}],"oa_status":"bronze","license":"http://www.biologists.com/user-licence-1-1","oa_locations":[{"url":"https://jcs.biologists.org/content/joces/129/2/430.full.pdf","host_type":"journal"},{"url":"https://jcs.biologists.org/content/joces/129/2/430.full.pdf","host_type":"BRONZE"},{"url":"https://jcs.biologists.org/content/joces/129/2/430.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1242/jcs.179200","host_type":"publisher"},{"url":"http://journals.biologists.com/jcs/article-pdf/129/2/430/1943999/jcs179200.pdf","host_type":"publisher"},{"url":"http://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.179200/2048535/jcs_179200v1.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/jcs.179200","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26631487","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4732290","host_type":"repository"},{"url":"http://jcs.biologists.org/cgi/content/short/129/2/430","host_type":"repository"}],"fields_of_study":["Cellular transport and secretion","Microtubule and mitosis dynamics","Photosynthetic Processes and Mechanisms","Biology","Medicine","Animals","Biological Transport","Cell Shape","Cytokinesis","Drosophila Proteins","Drosophila melanogaster","Female","Larva","Male","Nerve Tissue Proteins","Spindle Apparatus","Transport Vesicles"],"mesh_terms":["Animals","Biological Transport","Drosophila melanogaster","Female","Larva","Male","Spindle Apparatus","Nerve Tissue Proteins","Transport Vesicles","Drosophila Proteins","Cell Shape","Cytokinesis"],"keywords":["Cytokinesis","Ingression","Cleavage furrow","Cell biology","Biology","Septin","Midbody","Exocytosis","Mitosis","Vesicle","Cell division","Cell","Genetics","Membrane","Rop","Vesicle Trafficking","Sec1/munc18"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-13T19:58:33.384606Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}