{"doi":"10.1242/jcs.112904","title":"Clathrin is not required for SNX-BAR-retromer-mediated carrier formation","abstract":"<jats:title>Summary</jats:title>\n               <jats:p>Clathrin has been implicated in retromer-mediated trafficking, but its precise function remains elusive. Given the importance of retromers for efficient endosomal sorting, we have sought to clarify the relationship between clathrin and the SNX-BAR retromer. We find that the retromer SNX-BARs do not interact directly or indirectly with clathrin. In addition, we observe that SNX-BAR-retromer tubules and carriers are not clathrin coated. Furthermore, perturbing clathrin function, by overexpressing a dominant-negative clathrin or through suppression of clathrin expression, has no detectable effect on the frequency of SNX-BAR-retromer tubulation. We propose that SNX-BAR-retromer-mediated membrane deformation and carrier formation does not require clathrin, and hence the role of clathrin in SNX-BAR-retromer function would appear to lie in pre-SNX-BAR-retromer cargo sorting.</jats:p>","journal":"Journal of Cell Science","year":2013,"id":24440,"datarank":1.2344288065788078,"base_score":3.5263605246161616,"endowment":3.5263605246161616,"self_citation_contribution":0.5289540786924243,"citation_network_contribution":0.7054747278863835,"self_endowment_contribution":0.5289540786924243,"citer_contribution":0.7054747278863835,"corpus_percentile":null,"corpus_rank":null,"citation_count":33,"citer_count":28,"citers_with_citation_signal":26,"citers_with_endowment":26,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":145437,"name":"Peter J. Cullen","orcid":null,"position":1,"is_corresponding":false},{"id":146612,"name":"Ian J. McGough","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.5263605246161616,"endowment":3.5263605246161616,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23015596","pmcid":"PMC3603510","openalex_id":"https://openalex.org/W1978636875","authors":[],"funders":[{"funder_name":"Wellcome Trust","grant_id":"086777","title":null},{"funder_name":"Wellcome Trust","grant_id":"085743","title":null},{"funder_name":"Wellcome Trust","grant_id":"086777/Z/08/2","title":null},{"funder_name":"Wellcome Trust","grant_id":"089928/Z/09/Z","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null}],"total_grants":6,"fwci":1.687,"citation_percentile":0.81521789,"influential_citations":3,"citation_trend":[{"year":2013,"count":3},{"year":2014,"count":5},{"year":2015,"count":4},{"year":2016,"count":1},{"year":2017,"count":3},{"year":2018,"count":4},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2022,"count":3},{"year":2023,"count":3},{"year":2024,"count":4},{"year":2026,"count":1}],"oa_status":"bronze","license":"http://www.biologists.com/user-licence-1-1","oa_locations":[{"url":"https://jcs.biologists.org/content/joces/126/1/45.full.pdf","host_type":"journal"},{"url":"https://jcs.biologists.org/content/joces/126/1/45.full.pdf","host_type":"BRONZE"},{"url":"https://jcs.biologists.org/content/joces/126/1/45.full.pdf","host_type":"publisher"},{"url":"https://journals.biologists.com/jcs/article-pdf/126/1/45/2117424/jcs-126-01-0045.pdf","host_type":"publisher"},{"url":"http://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.112904/2044229/jcs112904.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/jcs.112904","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23015596","host_type":"repository"},{"url":"https://research-information.bris.ac.uk/en/publications/64e0bc45-c55f-4bef-9e2a-791e9ca06564","host_type":"repository"},{"url":"http://europepmc.org/articles/PMC3603510","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3603510","host_type":"repository"}],"fields_of_study":["Cellular transport and secretion","Retinal Development and Disorders","Retinal and Macular Surgery","Biology","Medicine","Clathrin","HeLa Cells","Humans","Immunoprecipitation","RNA Interference","Vesicular Transport Proteins"],"mesh_terms":["Clathrin","HeLa Cells","Humans","Vesicular Transport Proteins","RNA Interference","Immunoprecipitation","Hela Cells"],"keywords":["Retromer","Clathrin","Endosome","Cell biology","Biology","Endocytosis","Biochemistry","Receptor","Intracellular"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-07T22:25:08.927357Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}