{"doi":"10.1242/jcs.096495","title":"TIG3 Interaction at the Centrosome Alters Microtubule Distribution and Centrosome Function","abstract":"<jats:p>TIG3 is an importantpro-differentiation regulator that is expressed in the suprabasal epidermis. We have shown that TIG3 activates select keratinocyte differentiation-associated processesleading to cornified envelop formation. However, TIG3 also suppresses cell proliferation by an unknown mechanism. Our present studies suggest that growth cessation may be mediated via the impact of TIG3 on the centrosome and on microtubules. The centrosome regulates microtubule function in interphase cells and microtubule spindle formation in mitotic cells. We show that TIG3 co-localizes with γ-tubulin and pericentrin at the centrosome. TIG3 localization at the centrosome alters microtubule nucleation and reduces anterograde microtubule growth, increases acetylation and detyrosination of α-tubulin, increases insoluble tubulin and drives formation of a peripheral microtubule ring adjacent the plasma membrane. In addition, TIG3 suppresses centrosome separation, but not duplication, and reduces cell proliferation. We propose that TIG3 may regulate formation of the peripheral microtubule ring observed in keratinocytes in differentiated epidermis and also play a role in proliferation cessation in these cells.</jats:p>","journal":"Journal of Cell Science","year":2012,"id":589515,"datarank":0.6836593919411867,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.2989169883219562,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.2989169883219562,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":7,"citers_with_citation_signal":7,"citers_with_endowment":7,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":603670,"name":"Haibing Jiang","orcid":"0000-0003-3291-3210","position":1,"is_corresponding":false},{"id":1508294,"name":"Stuart Martin","orcid":null,"position":2,"is_corresponding":false},{"id":732487,"name":"Richard L. 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TIG3 localization at the centrosome alters microtubule nucleation and reduces anterograde microtubule growth, increases acetylation and detyrosination of α-tubulin, increases insoluble tubulin and drives formation of a peripheral microtubule ring adjacent the plasma membrane. In addition, TIG3 suppresses centrosome separation, but not duplication, and reduces cell proliferation. We propose that TIG3 may regulate formation of the peripheral microtubule ring observed in keratinocytes in differentiated epidermis and also play a role in proliferation cessation in these cells.</jats:p>","is_dataset_classified":null,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22427689","pmcid":"PMC3403232","openalex_id":"https://openalex.org/W1970141464","authors":[],"funders":[{"funder_name":"NIAMS NIH HHS","grant_id":"R01 AR049713","title":null},{"funder_name":"NIAMS NIH HHS","grant_id":"R01 AR49713","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P30 CA134274","title":null}],"total_grants":3,"fwci":1.2903,"citation_percentile":0.76236339,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":2},{"year":2014,"count":5},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2020,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"http://journals.biologists.com/jcs/article-pdf/125/11/2604/1927104/2604.pdf","host_type":"publisher"},{"url":"http://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.096495/2006904/jcs096495.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/jcs.096495","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22427689","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.932.284","host_type":""},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3403232","host_type":"repository"}],"fields_of_study":["Microtubule and mitosis dynamics","Skin and Cellular Biology Research","Plant Reproductive Biology","Cell Division","Centrosome","Humans","Keratinocytes","Male","Microtubules","Models, Biological","Nocodazole","Protein Binding","Protein Transport","Receptors, Retinoic Acid","Tubulin"],"mesh_terms":["Cell Division","Humans","Male","Microtubules","Models, Biological","Protein Binding","Tubulin","Keratinocytes","Nocodazole","Receptors, Retinoic Acid","Centrosome","Protein Transport"],"keywords":["Centrosome","Cell biology","Biology","Microtubule nucleation","Microtubule","Centrosome cycle","Microtubule organizing center","Mitosis","Tubulin","Cell","Cell cycle","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-23T20:28:47.636411Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}