{"doi":"10.1242/jcs.073486","title":"Microtubule organization in the final stages of cytokinesis as revealed by cryo-electron tomography","abstract":"<jats:p>The completion of cytokinesis is dominated by the midbody, a tightly-packed microtubule (MT)-based bridge that transiently connects the two daughter cells. Assembled from condensed, spindle-MTs and numerous associated proteins, the midbody gradually narrows down until daughter cell partitioning occurs at this site. Although described many years ago, detailed understanding of the abscission process remains lacking. Applying cryo-electron tomography to purified midbodies, in combination with fluorescence microscopy, we present here new insight into MT organization within the midbody. We find that the midbody is spatially divided into a core bundle of MTs that traverses the electron-dense overlap region (continuous MTs), surrounded by MTs that terminate within the overlap region (polar MTs). Residual continuous MTs remained intact up to the verge of abscission, whereas the residual polar MTs lost their organization and retreated from the overlap region at late cytokinesis stages. A detailed localization of the microtubule-bundling protein PRC1 supports the above notion. Our study thus provides a detailed account of the abscission process and suggests that the midbody, having acquired a distinct MT architecture as compared to the preceding central spindle, actively facilitates the final stage of cytokinesis.</jats:p>","journal":"Journal of Cell Science","year":2011,"id":42019,"datarank":2.02764472618577,"base_score":3.7376696182833684,"endowment":3.7376696182833684,"self_citation_contribution":0.5606504427425053,"citation_network_contribution":1.466994283443265,"self_endowment_contribution":0.5606504427425053,"citer_contribution":1.466994283443265,"corpus_percentile":null,"corpus_rank":null,"citation_count":41,"citer_count":35,"citers_with_citation_signal":34,"citers_with_endowment":34,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":48.75,"fair_percentile":44.94283201407212,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":201656,"name":"Shahar Abramovitch","orcid":null,"position":1,"is_corresponding":false},{"id":201657,"name":"Helena Sabanay","orcid":null,"position":2,"is_corresponding":false},{"id":201658,"name":"Ohad Medalia","orcid":null,"position":3,"is_corresponding":false},{"id":201655,"name":"Nadav Elad","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.7376696182833684,"endowment":3.7376696182833684,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21187346","pmcid":null,"openalex_id":"https://openalex.org/W2057631687","authors":[],"funders":[{"funder_name":"European Research Council","grant_id":"243047","title":"Revealing the molecular architecture of integrin mediated cell adhesion"}],"total_grants":1,"fwci":2.5515,"citation_percentile":0.88664567,"influential_citations":1,"citation_trend":[{"year":2012,"count":6},{"year":2013,"count":7},{"year":2014,"count":4},{"year":2015,"count":2},{"year":2016,"count":4},{"year":2018,"count":2},{"year":2019,"count":3},{"year":2021,"count":3},{"year":2022,"count":2},{"year":2023,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":"other-oa","oa_locations":[{"url":"https://www.zora.uzh.ch/id/eprint/48189/12/Elad_et_al_Microtubule-V.pdf","host_type":"GREEN"},{"url":"http://journals.biologists.com/jcs/article-pdf/124/2/207/1560679/207.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/jcs.073486","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21187346","host_type":"repository"},{"url":"https://doi.org/10.5167/uzh-48189","host_type":"repository"},{"url":"http://jcs.biologists.org/content/124/2/207.full.pdf","host_type":""},{"url":"https://dx.doi.org/10.5167/uzh-48189","host_type":""},{"url":"https://dx.doi.org/10.1242/jcs.073486","host_type":""},{"url":"https://www.zora.uzh.ch/id/eprint/48189/","host_type":""},{"url":"http://dx.doi.org/10.1242/jcs.073486","host_type":""}],"fields_of_study":["Microtubule and mitosis dynamics","Photosynthetic Processes and Mechanisms","Protist diversity and phylogeny","Medicine","Biology","Materials Science","0301 basic medicine","03 medical and health sciences","Animals","CHO Cells","Cell Line","Cells","Cricetinae","Cricetulus","Cytokinesis","Electron Microscope Tomography","Microtubules","Protein Transport"],"mesh_terms":["Animals","Cell Line","Cells","Cricetulus","Cricetinae","Microtubules","CHO Cells","Protein Transport","Cytokinesis","Electron Microscope Tomography"],"keywords":["Midbody","Cytokinesis","Abscission","Biology","Cryo-electron tomography","Microtubule","Cell biology","Electron tomography","Cell division","Cell","Botany","Electron microscope","Genetics","Tomography","Scanning transmission electron microscopy","Physics","Optics","Electron Microscope Tomography","Cells","CHO Cells","Microtubules","Cell Line","1307 Cell Biology","Protein Transport","Cricetulus","Cricetinae","10019 Department of Biochemistry","Animals","570 Life sciences; 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