{"doi":"10.1242/jcs.033233","title":"Progression of meiotic recombination requires structural maturation of the central element of the synaptonemal complex","abstract":"<jats:p>The synaptonemal complex is an elaborate meiosis-specific supramolecular protein assembly that promotes chromosome synapsis and meiotic recombination. We inactivated the meiosis-specific gene Tex12 and found that TEX12 is essential for progression of meiosis in both male and female germ cells. Structural analysis of the synaptonemal complex in Tex12–/– meiocytes revealed a disrupted central element structure, a dense structure residing between the synapsed homologous chromosomes. Chromosome synapsis is initiated at multiple positions along the paired homologous chromosomes in Tex12–/– meiotic cells, but fails to propagate along the chromosomes. Furthermore, although meiotic recombination is initiated in Tex12–/– meiotic cells, these early recombination events do not develop into meiotic crossovers. Hence, the mere initiation of synapsis is not sufficient to support meiotic crossing-over. Our results show that TEX12 is a component of the central element structure of the synaptonemal complex required for propagation of synapsis along the paired homologous chromosomes and maturation of early recombination events into crossovers.</jats:p>","journal":"Journal of Cell Science","year":2008,"id":590185,"datarank":4.324498774998569,"base_score":4.897839799950911,"endowment":4.897839799950911,"self_citation_contribution":0.7346759699926367,"citation_network_contribution":3.5898228050059315,"self_endowment_contribution":0.7346759699926367,"citer_contribution":3.5898228050059315,"corpus_percentile":null,"corpus_rank":null,"citation_count":133,"citer_count":107,"citers_with_citation_signal":97,"citers_with_endowment":97,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":598781,"name":"Hong Wang","orcid":"0000-0002-0213-3523","position":1,"is_corresponding":false},{"id":1510054,"name":"Ewelina Bolcun-Filas","orcid":null,"position":2,"is_corresponding":false},{"id":1510055,"name":"Howard J. Cooke","orcid":null,"position":3,"is_corresponding":false},{"id":1510056,"name":"Ricardo Benavente","orcid":null,"position":4,"is_corresponding":false},{"id":1510057,"name":"Christer Höög","orcid":null,"position":5,"is_corresponding":false},{"id":1510053,"name":"Geert Hamer","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Progression of meiotic recombination requires structural maturation of the central element of the synaptonemal complex","abstract":"<jats:p>The synaptonemal complex is an elaborate meiosis-specific supramolecular protein assembly that promotes chromosome synapsis and meiotic recombination. We inactivated the meiosis-specific gene Tex12 and found that TEX12 is essential for progression of meiosis in both male and female germ cells. Structural analysis of the synaptonemal complex in Tex12–/– meiocytes revealed a disrupted central element structure, a dense structure residing between the synapsed homologous chromosomes. Chromosome synapsis is initiated at multiple positions along the paired homologous chromosomes in Tex12–/– meiotic cells, but fails to propagate along the chromosomes. Furthermore, although meiotic recombination is initiated in Tex12–/– meiotic cells, these early recombination events do not develop into meiotic crossovers. Hence, the mere initiation of synapsis is not sufficient to support meiotic crossing-over. Our results show that TEX12 is a component of the central element structure of the synaptonemal complex required for propagation of synapsis along the paired homologous chromosomes and maturation of early recombination events into crossovers.</jats:p>","is_dataset_classified":null,"base_score":4.897839799950911,"endowment":4.897839799950911,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18611960","pmcid":null,"openalex_id":"https://openalex.org/W2057965747","authors":[],"funders":[],"total_grants":0,"fwci":3.5088,"citation_percentile":0.93265785,"influential_citations":0,"citation_trend":[{"year":2012,"count":11},{"year":2013,"count":3},{"year":2014,"count":3},{"year":2015,"count":1},{"year":2016,"count":11},{"year":2017,"count":6},{"year":2018,"count":6},{"year":2019,"count":8},{"year":2020,"count":10},{"year":2021,"count":14},{"year":2022,"count":10},{"year":2023,"count":9},{"year":2024,"count":2},{"year":2025,"count":8},{"year":2026,"count":4}],"oa_status":"closed","license":null,"oa_locations":[{"url":"http://journals.biologists.com/jcs/article-pdf/121/15/2445/1562398/2445.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/jcs.033233","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18611960","host_type":"repository"},{"url":"https://pure.amsterdamumc.nl/en/publications/8f4a0b90-6e07-41f1-8756-bc847f15be50","host_type":"repository"},{"url":"https://pure.amc.nl/en/publications/progression-of-meiotic-recombination-requires-structural-maturation-of-the-central-element-of-the-synaptonemal-complex(2f20699c-86bf-45f1-9402-97ab323f7eb8).html","host_type":"repository"}],"fields_of_study":["DNA Repair Mechanisms","Genomics and Chromatin Dynamics","Microtubule and mitosis dynamics"],"mesh_terms":["Animals","Chromosomal Proteins, Non-Histone","Crossing Over, Genetic","Immunohistochemistry","Meiosis","Mice, Transgenic","Recombination, Genetic","Synaptonemal Complex","Mice","DNA Breaks, Double-Stranded"],"keywords":["Synapsis","Synaptonemal complex","Meiosis","Homologous chromosome","Biology","Chromosomal crossover","Homologous recombination","Meiocyte","Genetic recombination","Genetics","Bivalent (engine)","Chromosome","Cell biology","Recombination","Gene","Chemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-24T14:19:19.278094Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}