{"doi":"10.1242/jcs.003111","title":"The retromer component sorting nexin-1 is required for efficient retrograde transport of Shiga toxin from early endosome to the trans Golgi network","abstract":"<jats:p>The mammalian retromer complex is a multi-protein complex that regulates retrograde transport of the cation-independent mannose 6-phosphate receptor (CI-MPR) from early endosomes to the trans Golgi network (TGN). It consists of two subcomplexes: a membrane-bound coat comprising sorting nexin-1 (SNX1) and possibly sorting nexin-2 (SNX2), and a cargo-selective subcomplex, composed of VPS26, VPS29 and VPS35. In addition to the retromer, a variety of other protein complexes has been suggested to regulate endosome-to-TGN transport of not only the CI-MPR but a wide range of other cargo proteins. Here, we have examined the role of SNX1 and SNX2 in endosomal sorting of Shiga and cholera toxins, two toxins that undergo endosome-to-TGN transport en route to their cellular targets located within the cytosol. By using small interfering RNA (siRNA)-mediated silencing combined with single-cell fluorescent-toxin-uptake assays and well-established biochemical assays to analyze toxin delivery to the TGN, we have established that suppression of SNX1 leads to a significant reduction in the efficiency of endosome-to-TGN transport of the Shiga toxin B-subunit. Furthermore, we show that for the B subunit of cholera toxin, retrograde endosome-to-TGN transport is less reliant upon SNX1. Overall, our data establish a role for SNX1 in the endosome-to-TGN transport of Shiga toxin and are indicative for a fundamental difference between endosomal sorting of Shiga and cholera toxins into endosome-to-TGN retrograde transport pathways.</jats:p>","journal":"Journal of Cell Science","year":2007,"id":24026,"datarank":4.6706305993133705,"base_score":4.77912349311153,"endowment":4.77912349311153,"self_citation_contribution":0.7168685239667295,"citation_network_contribution":3.953762075346641,"self_endowment_contribution":0.7168685239667295,"citer_contribution":3.953762075346641,"corpus_percentile":null,"corpus_rank":null,"citation_count":118,"citer_count":100,"citers_with_citation_signal":90,"citers_with_endowment":90,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":145435,"name":"Vincent Popoff","orcid":null,"position":1,"is_corresponding":false},{"id":145436,"name":"Ludger Johannes","orcid":null,"position":2,"is_corresponding":false},{"id":145437,"name":"Peter J. 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Bujny","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.77912349311153,"endowment":4.77912349311153,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17550970","pmcid":null,"openalex_id":"https://openalex.org/W2083045229","authors":[],"funders":[{"funder_name":"Wellcome Trust","grant_id":"","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null}],"total_grants":2,"fwci":5.3996,"citation_percentile":0.96505996,"influential_citations":9,"citation_trend":[{"year":2012,"count":14},{"year":2013,"count":9},{"year":2014,"count":7},{"year":2015,"count":4},{"year":2016,"count":4},{"year":2017,"count":2},{"year":2018,"count":4},{"year":2019,"count":1},{"year":2020,"count":6},{"year":2021,"count":4},{"year":2022,"count":5},{"year":2023,"count":2},{"year":2024,"count":2},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"public-domain","oa_locations":[{"url":"http://jcs.biologists.org/content/120/12/2010.full.pdf","host_type":"BRONZE"},{"url":"http://journals.biologists.com/jcs/article-pdf/120/12/2010/1510957/2010.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/jcs.003111","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17550970","host_type":"repository"}],"fields_of_study":["Cellular transport and secretion","RNA Interference and Gene Delivery","Lipid Membrane Structure and Behavior","Biology","Medicine","Animals","Biological Transport","Carrier Proteins","Cholera Toxin","Endosomes","HeLa Cells","Humans","Multiprotein Complexes","Protein Isoforms","RNA Interference","Shiga Toxin","Sorting Nexins","Vesicular Transport Proteins","trans-Golgi Network"],"mesh_terms":["Animals","Biological Transport","Carrier Proteins","Cholera Toxin","HeLa Cells","Humans","Endosomes","Protein Isoforms","trans-Golgi Network","Shiga Toxin","Vesicular Transport Proteins","RNA Interference","Multiprotein Complexes","Sorting Nexins","Hela Cells"],"keywords":["Endosome","Retromer","Sorting nexin","Cell biology","Cholera toxin","Biology","Transport protein","Golgi apparatus","Shiga toxin","Biochemistry","Microbiology","Intracellular","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-07T21:14:43.503022Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}