{"doi":"10.1242/dev.33.4.947","title":"Nicotinamide adenine dinucleotide levels in chick limb mesodermal cells <i>in vitro:</i> effects of 3-acetylpyridine and nicotinamide","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>The studies reported here show that in cultures of developing mesodermal cells, chondrogenic expression is associated with a progressive decline in cellular NAD+ levels. Furthermore, reduced cellular NAD+ levels resulting from exposure to the nicotinamide analog 3-acetyl-pyridine are correlated with a 2- to 100-fold potentiation of chondrogenic expression. Conversely, elevated NAD+ levels resulting from exposure to nicotinamide alone are correlated with inhibition of chondrogenic expression. These data are consistent with the hypothesis that pyridine nucleotides, or some derivative thereof, play a central role in the control of muscle and cartilage development in embryonic chick limbs.</jats:p>","journal":"Development","year":1975,"id":626672,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":44666,"name":"Arnold I. Caplan","orcid":"0000-0002-8677-6621","position":1,"is_corresponding":false},{"id":1621136,"name":"Martin J. Rosenberg","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Nicotinamide adenine dinucleotide levels in chick limb mesodermal cells <i>in vitro:</i> effects of 3-acetylpyridine and nicotinamide","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>The studies reported here show that in cultures of developing mesodermal cells, chondrogenic expression is associated with a progressive decline in cellular NAD+ levels. Furthermore, reduced cellular NAD+ levels resulting from exposure to the nicotinamide analog 3-acetyl-pyridine are correlated with a 2- to 100-fold potentiation of chondrogenic expression. Conversely, elevated NAD+ levels resulting from exposure to nicotinamide alone are correlated with inhibition of chondrogenic expression. These data are consistent with the hypothesis that pyridine nucleotides, or some derivative thereof, play a central role in the control of muscle and cartilage development in embryonic chick limbs.</jats:p>","is_dataset_classified":null,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"170357","pmcid":null,"openalex_id":"https://openalex.org/W2436550407","authors":[],"funders":[],"total_grants":0,"fwci":0.2117,"citation_percentile":0.53357322,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"http://www.biologists.com/user-licence-1-1/","oa_locations":[{"url":"https://journals.biologists.com/dev/article-pdf/33/4/947/2666346/develop_33_4_947.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/dev.33.4.947","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/170357","host_type":"repository"}],"fields_of_study":["RNA Interference and Gene Delivery","Antimicrobial Peptides and Activities","Virus-based gene therapy research"],"mesh_terms":["Animals","Cells, Cultured","Chick Embryo","DNA","Mesoderm","NAD","Niacinamide","Pyridines"],"keywords":["NAD+ kinase","Biology","Nicotinamide","Chondrogenesis","Nicotinamide adenine dinucleotide","Nicotinamide phosphoribosyltransferase","Cartilage","In vitro","Embryo","Cell biology","Biochemistry","Endocrinology","Enzyme","Anatomy"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T14:46:58.151073Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}