{"doi":"10.1242/dev.204964","title":"Defects in nephrogenesis result in an expansion of the <i>Foxd1</i> + stromal progenitor population","abstract":"The Foxd1+ stromal progenitor cells give rise to the majority of the renal interstitium; yet, much remains to be understood about how this self-renewing progenitor population is regulated during development. Here, we demonstrate that disruption of the nephron progenitor cell (NPC) lineage via loss of Wt1 (i.e. Six2cre;Wt1c/c) results in an expansion of Foxd1+ progenitor cells in mice. Analyses of two additional models (i.e. Wnt4-null mutants, which fail to form nephron structures similar to Six2cre;Wt1c/c kidneys, and NPC ablation via diphtheria toxin using the Six2cre;RosaDTAc/+) phenocopy the expansion in Foxd1+ cells and further confirm that mutant kidneys with defects in nephrogenesis develop an abnormal increase in the stromal progenitor population. Furthermore, single nuclei RNA-sequencing shows transcriptional changes in the Foxd1+ progenitor cells from Six2cre;Wt1c/c kidneys and identifies a distinct subcluster of the Foxd1+ stroma, which is maintained independent of signals from the nephrogenic niche in the Six2cre;RosaDTAc/+ model. Overall, these findings provide insights into the developmental regulation of the stromal progenitor population and uncover heterogeneity within the Foxd1+ cells, which undergo both cellular and molecular changes in response to defects in nephrogenesis.","journal":"Development","year":2025,"id":537459,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9525,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":314002,"name":"Yan Liu","orcid":"0000-0003-4242-4840","position":1,"is_corresponding":false},{"id":1423251,"name":"D. Raju","orcid":"0000-0002-2183-7841","position":2,"is_corresponding":false},{"id":690480,"name":"John T. Lafin","orcid":"0000-0003-2335-8999","position":3,"is_corresponding":false},{"id":1423252,"name":"Yanru Ma","orcid":"0000-0002-1678-8985","position":4,"is_corresponding":false},{"id":502023,"name":"Dhruv Gaur","orcid":null,"position":5,"is_corresponding":false},{"id":1423708,"name":"S. Khadka","orcid":null,"position":6,"is_corresponding":false},{"id":240348,"name":"Chao Xing","orcid":"0000-0002-1838-0502","position":7,"is_corresponding":false},{"id":323733,"name":"Andrew P. McMahon","orcid":"0000-0002-3779-1729","position":8,"is_corresponding":false},{"id":284866,"name":"Thomas J. Carroll","orcid":"0000-0002-8322-4928","position":9,"is_corresponding":false},{"id":244960,"name":"Keri A. Drake","orcid":"0000-0002-9355-5202","position":10,"is_corresponding":false},{"id":1222455,"name":"Michael G. Michalopulos","orcid":null,"position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":null,"created_at":"2026-07-19T02:52:12.997494Z","pmid":"41358813","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}