{"doi":"10.1242/dev.204872","title":"Compensatory action of different types of <i>cis</i> -regulatory elements buffers the striped expression of <i>Drosophila</i> pair-rule genes","abstract":"The striped expression of pair-rule genes in Drosophila embryos is a paradigm for understanding transcriptional control of development. Pair-rule striped expression is regulated by two types of cis-regulatory elements: stripe-specific elements respond to non-periodic cues in different regions of the embryo to establish individual stripes while 7-stripe elements simultaneously regulate all stripes, responding to pair-rule genes expressed in stripes. Here, we assess roles of stripe-specific versus 7-stripe elements for the pair-rule gene ftz. We show that loss of a ftz stripe 2 element is compensated by 7-stripe elements, even though they respond to different spatiotemporal cues. We next investigate whether similar rules apply to the classic eve stripe2 element. Animals homozygous for a genomic deletion of eve stripe2 are viable and fertile; stripe 2 expression is perturbed early but re-establishes sufficiently to regulate downstream target genes. However, temperature or genetic stress decrease viability of ftz and eve stripe 2 deletion mutants. Thus, these stripe-specific elements contribute to robustness but are not absolutely required for segment formation. Two separate routes to establishing stripes, stripe-specific and 7-stripe elements, buffer each other, adding complexity to embryonic patterning.","journal":"Development","year":2025,"id":554520,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9574,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1285579,"name":"K M Au","orcid":null,"position":1,"is_corresponding":false},{"id":755100,"name":"Minh Đức Lê","orcid":"0000-0002-2953-2815","position":2,"is_corresponding":false},{"id":794409,"name":"Patricia Graham","orcid":null,"position":3,"is_corresponding":false},{"id":350317,"name":"Leslie Pick","orcid":"0000-0002-4505-5107","position":4,"is_corresponding":false},{"id":793779,"name":"Matthew D. Fischer","orcid":"0000-0002-3266-3541","position":0,"is_corresponding":true}],"reference_count":90,"raw_metadata":null,"created_at":"2026-07-19T02:54:50.112989Z","pmid":"41293990","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}