{"doi":"10.1242/dev.204513","title":"Sex-biased gene expression precedes sexual dimorphism in the agonadal annelid <i>Platynereis dumerilii</i>","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>Gametogenesis is the process by which germ cells differentiate into mature sperm and oocytes – cells that are essential for sexual reproduction. The sex-specific molecular programs that drive spermatogenesis and oogenesis can also serve as sex identification markers. Platynereis dumerilii is a research organism that has been studied in many areas of developmental biology. However, investigations often disregard sex, as P. dumerilii juveniles lack sexual dimorphism. The molecular mechanisms of gametogenesis in the segmented worm P. dumerilii are also largely unknown. In this study, we used RNA sequencing to investigate the transcriptomic profiles of gametogenesis in P. dumerilii juveniles. Our analysis revealed that sex-biased gene expression becomes increasingly pronounced during the advanced developmental stages, as worms approach maturation. We identified conserved genes associated with spermatogenesis, such as dmrt1, and with oogenesis, such as the previously unidentified gene psmt. Additionally, putative long non-coding RNAs were upregulated in both male and female gametogenic programs. This study provides a foundational resource for germ cell research in P. dumerilii and markers for sex identification, and offers comparative data to enhance our understanding of the evolution of gametogenesis mechanisms across species.</jats:p>","journal":"Development","year":2025,"id":655190,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":621390,"name":"Ryan W. Null","orcid":"0000-0002-3830-4152","position":1,"is_corresponding":false},{"id":621392,"name":"B. Duygu Özpolat","orcid":"0000-0002-1900-965X","position":2,"is_corresponding":false},{"id":1710170,"name":"Rannyele P. Ribeiro","orcid":"0000-0002-0304-7053","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Sex-biased gene expression precedes sexual dimorphism in the agonadal annelid <i>Platynereis dumerilii</i>","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>Gametogenesis is the process by which germ cells differentiate into mature sperm and oocytes – cells that are essential for sexual reproduction. The sex-specific molecular programs that drive spermatogenesis and oogenesis can also serve as sex identification markers. Platynereis dumerilii is a research organism that has been studied in many areas of developmental biology. However, investigations often disregard sex, as P. dumerilii juveniles lack sexual dimorphism. The molecular mechanisms of gametogenesis in the segmented worm P. dumerilii are also largely unknown. In this study, we used RNA sequencing to investigate the transcriptomic profiles of gametogenesis in P. dumerilii juveniles. Our analysis revealed that sex-biased gene expression becomes increasingly pronounced during the advanced developmental stages, as worms approach maturation. We identified conserved genes associated with spermatogenesis, such as dmrt1, and with oogenesis, such as the previously unidentified gene psmt. Additionally, putative long non-coding RNAs were upregulated in both male and female gametogenic programs. This study provides a foundational resource for germ cell research in P. dumerilii and markers for sex identification, and offers comparative data to enhance our understanding of the evolution of gametogenesis mechanisms across species.</jats:p>","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"40067261","pmcid":"PMC12045600","openalex_id":"https://openalex.org/W4408323034","authors":[],"funders":[{"funder_name":"National Institute of General Medical Sciences","grant_id":"1R35GM138008-01","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R35 GM138008","title":null},{"funder_name":"Hibbitt Fellowship","grant_id":"","title":null},{"funder_name":"Washington University in St. Louis Startup funds","grant_id":"","title":null},{"funder_name":"Washington University in St Louis","grant_id":"","title":null},{"funder_name":"Hibbitt Fellowship","grant_id":"","title":null},{"funder_name":"Washington University in St Louis","grant_id":"","title":null},{"funder_name":"Washington University in St. Louis Startup funds","grant_id":"","title":null}],"total_grants":8,"fwci":1.8307,"citation_percentile":0.82325901,"influential_citations":0,"citation_trend":[{"year":2025,"count":2}],"oa_status":"green","license":"http://www.biologists.com/user-licence-1-1/","oa_locations":[{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12045600/","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12045600/","host_type":"repository"},{"url":"https://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.204513/3638256/dev204513.pdf","host_type":"publisher"},{"url":"https://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.204513/3644891/dev204513.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/dev.204513","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/40067261","host_type":"repository"}],"fields_of_study":["Parasite Biology and Host Interactions","Genetics, Aging, and Longevity in Model Organisms","Invertebrate Taxonomy and Ecology","Animals","Female","Male","Sex Characteristics","Gene Expression Regulation, Developmental","Oogenesis","Spermatogenesis","Transcriptome","Polychaeta","RNA, Long Noncoding","Gametogenesis","Gene Expression Profiling"],"mesh_terms":["Animals","Female","Gametogenesis","Male","Oogenesis","Polychaeta","Sex Characteristics","Spermatogenesis","Gene Expression Regulation, Developmental","Gene Expression Profiling","Transcriptome","RNA, Long Noncoding"],"keywords":["Biology","Gametogenesis","Oogenesis","Sexual dimorphism","Annelid","Gene","Doublesex","Transcriptome","Genetics","Developmental biology","Evolutionary biology","Gene expression","Zoology","RNA","Embryogenesis","Reproduction","Germline","Sexual differentiation","Transcriptomics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"bioproject"},{"name":"gen"},{"name":"doi"},{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-11T09:59:48.425147Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}