{"doi":"10.1242/dev.202694","title":"A role for BYN-1/bystin in cellular uptake and clearance of residual bodies in the <i>Caenorhabditis elegans</i> germline","abstract":"GLH/Vasa/DDX4 helicases are core germ-granule proteins that promote germline development and fertility. A yeast-two-hybrid screen using Caenorhabditis elegans GLH-1 as bait identified BYN-1, the homolog of human bystin/BYSL. In humans, bystin promotes cell adhesion and invasion in gliomas, and, with its binding partner trophinin, triggers embryonic implantation into the uterine wall. C. elegans embryos do not implant and lack a homolog of trophinin, but both trophinin and GLH-1 contain unique decapeptide phenylalanine-glycine (FG)-repeat domains. In germ cells, we find endogenous BYN-1 in the nucleolus, partitioned away from cytoplasmic germ granules. However, BYN-1 enters the cytoplasm during spermatogenesis to colocalize with GLH-1. Both proteins become deposited in residual bodies (RBs), which are then engulfed and cleared by the somatic gonad. We show that BYN-1 acts upstream of CED-1 to drive RB engulfment, and that removal of the FG-repeat domains from GLH-1 and GLH-2 can partially phenocopy byn-1 defects in RB clearance. These results point to an evolutionarily conserved pathway whereby cellular uptake is triggered by the cytoplasmic mobilization of bystin/BYN-1 to interact with proteins harboring FG-repeats.","journal":"Development","year":2024,"id":477687,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9522,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":630359,"name":"Emily L. Spaulding","orcid":"0000-0001-9680-6348","position":1,"is_corresponding":false},{"id":570833,"name":"Catherine S. Sharp","orcid":"0000-0002-8459-1084","position":2,"is_corresponding":false},{"id":824663,"name":"Pankaj Garg","orcid":"0000-0002-5483-169X","position":3,"is_corresponding":false},{"id":1316321,"name":"Esther Jeon","orcid":null,"position":4,"is_corresponding":false},{"id":825276,"name":"Lyn S. Miranda Portillo","orcid":null,"position":5,"is_corresponding":false},{"id":1316322,"name":"Noah Lind","orcid":null,"position":6,"is_corresponding":false},{"id":570834,"name":"Dustin L. Updike","orcid":"0000-0002-1745-3669","position":7,"is_corresponding":false},{"id":501204,"name":"Hyemin Min","orcid":"0000-0001-9691-770X","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":null,"created_at":"2026-07-19T02:06:37.812633Z","pmid":"39377446","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}