{"doi":"10.1242/dev.202169","title":"Leveraging dominant-negative histone H3 K-to-M mutations to study chromatin during differentiation and development","abstract":"Histone modifications are associated with regulation of gene expression that controls a vast array of biological processes. Often, these associations are drawn by correlating the genomic location of a particular histone modification with gene expression or phenotype; however, establishing a causal relationship between histone marks and biological processes remains challenging. Consequently, there is a strong need for experimental approaches to directly manipulate histone modifications. A class of mutations on the N-terminal tail of histone H3, lysine-to-methionine (K-to-M) mutations, was identified as dominant-negative inhibitors of histone methylation at their respective and specific residues. The dominant-negative nature of K-to-M mutants makes them a valuable tool for studying the function of specific methylation marks on histone H3. Here, we review recent applications of K-to-M mutations to understand the role of histone methylation during development and homeostasis. We highlight important advantages and limitations that require consideration when using K-to-M mutants, particularly in a developmental context.","journal":"Development","year":2023,"id":359839,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9554,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":704473,"name":"Alison R. Swearingen","orcid":null,"position":1,"is_corresponding":false},{"id":269895,"name":"Mariel Coradin","orcid":"0000-0003-3560-4384","position":2,"is_corresponding":false},{"id":1110813,"name":"Mika Nevo","orcid":"0000-0002-2385-4784","position":3,"is_corresponding":false},{"id":1110814,"name":"Huong Thi Thanh Tran","orcid":"0009-0009-3486-1005","position":4,"is_corresponding":false},{"id":1111316,"name":"Emir Bajric","orcid":null,"position":5,"is_corresponding":false},{"id":72477,"name":"Justin Brumbaugh","orcid":"0000-0002-9605-2010","position":6,"is_corresponding":false},{"id":1111315,"name":"Ksenia Serdyukova","orcid":null,"position":0,"is_corresponding":true}],"reference_count":170,"raw_metadata":null,"created_at":"2026-07-19T01:13:57.361401Z","pmid":"37846748","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}