{"doi":"10.1242/dev.201755","title":"SIN-3 acts in distinct complexes to regulate the germline transcriptional program in <i>Caenorhabditis elegans</i>","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>The transcriptional co-regulator SIN3 influences gene expression through multiple interactions that include histone deacetylases. Haploinsufficiency and mutations in SIN3 are the underlying cause of Witteveen–Kolk syndrome and related intellectual disability and autism syndromes, emphasizing its key role in development. However, little is known about the diversity of its interactions and functions in developmental processes. Here, we show that loss of SIN-3, the single SIN3 homolog in Caenorhabditis elegans, results in maternal-effect sterility associated with de-regulation of the germline transcriptome, including de-silencing of X-linked genes. We identify at least two distinct SIN3 complexes containing specific histone deacetylases and show that they differentially contribute to fertility. Single-cell, single-molecule fluorescence in situ hybridization reveals that in sin-3 mutants the X chromosome becomes re-expressed prematurely and in a stochastic manner in individual germ cells, suggesting a role for SIN-3 in its silencing. Furthermore, we identify histone residues whose acetylation increases in the absence of SIN-3. Together, this work provides a powerful framework for the in vivo study of SIN3 and associated proteins.</jats:p>","journal":"Development","year":2023,"id":615727,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":757258,"name":"Matthieu Caron","orcid":"0000-0002-6905-7545","position":1,"is_corresponding":false},{"id":388016,"name":"Loïc Gely","orcid":"0009-0006-5976-1025","position":2,"is_corresponding":false},{"id":757259,"name":"Annie Adrait","orcid":"0000-0002-1565-2859","position":3,"is_corresponding":false},{"id":1587197,"name":"Victoria Pakulska","orcid":"0000-0001-7502-1111","position":4,"is_corresponding":false},{"id":92127,"name":"Yohann Couté","orcid":"0000-0003-3896-6196","position":5,"is_corresponding":false},{"id":1587198,"name":"Manon Chevalier","orcid":"0000-0002-7749-6898","position":6,"is_corresponding":false},{"id":347301,"name":"Christian G. Riedel","orcid":"0000-0002-3689-8022","position":7,"is_corresponding":false},{"id":1587199,"name":"Cecile Bedet","orcid":"0000-0003-3018-6240","position":8,"is_corresponding":false},{"id":388019,"name":"Francesca Palladino","orcid":"0000-0003-3087-449X","position":9,"is_corresponding":false},{"id":388014,"name":"Valérie Robert","orcid":"0000-0003-2811-4694","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"SIN-3 acts in distinct complexes to regulate the germline transcriptional program in <i>Caenorhabditis elegans</i>","abstract":"The transcriptional co-regulator SIN3 influences gene expression through multiple interactions that include histone deacetylases. Haploinsufficiency and mutations in SIN3 are the underlying cause of Witteveen-Kolk syndrome and related intellectual disability and autism syndromes, emphasizing its key role in development. However, little is known about the diversity of its interactions and functions in developmental processes. Here, we show that loss of SIN-3, the single SIN3 homolog in Caenorhabditis elegans, results in maternal-effect sterility associated with de-regulation of the germline transcriptome, including de-silencing of X-linked genes. We identify at least two distinct SIN3 complexes containing specific histone deacetylases and show that they differentially contribute to fertility. Single-cell, single-molecule fluorescence in situ hybridization reveals that in sin-3 mutants the X chromosome becomes re-expressed prematurely and in a stochastic manner in individual germ cells, suggesting a role for SIN-3 in its silencing. Furthermore, we identify histone residues whose acetylation increases in the absence of SIN-3. Together, this work provides a powerful framework for the in vivo study of SIN3 and associated proteins.","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37818613","pmcid":"PMC10617626","openalex_id":"https://openalex.org/W4387515607","authors":[],"funders":[{"funder_name":"Vetenskapsrådet","grant_id":"2017-06088","title":null},{"funder_name":"Novo Nordisk Fonden","grant_id":"NNF22°C0078353","title":null},{"funder_name":"Agence Nationale de la Recherche","grant_id":"19-CE12-0025-01","title":null},{"funder_name":"Novo Nordisk Fonden","grant_id":"NNF21°C0070427","title":null},{"funder_name":"Novo Nordisk Fonden","grant_id":"NNF22OC0078353","title":null},{"funder_name":"Vetenskapsrådet","grant_id":"2019-04868","title":null},{"funder_name":"Cancerfonden","grant_id":"20 1034 Pj","title":null},{"funder_name":"Novo Nordisk Fonden","grant_id":"NNF21OC0070427","title":null},{"funder_name":"Swedish Research Council","grant_id":"unidentified","title":"unidentified"},{"funder_name":"French National Research Agency (ANR)","grant_id":"ANR-17-EURE-0003","title":null},{"funder_name":"Centre National de la Recherche Scientifique","grant_id":"","title":null}],"total_grants":11,"fwci":0.3457,"citation_percentile":0.55821727,"influential_citations":0,"citation_trend":[{"year":2024,"count":3},{"year":2026,"count":2}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.201755/3231148/dev201755.pdf","host_type":"journal"},{"url":"https://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.201755/3231148/dev201755.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/dev.201755","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/37818613","host_type":"repository"},{"url":"https://pubmed.ncbi.nlm.nih.gov/38771303","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10617626","host_type":"repository"},{"url":"https://hal.science/hal-04237476","host_type":"repository"},{"url":"https://hal.science/hal-04277487","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC10617626","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1242/dev.201755","host_type":""},{"url":"https://hal.science/hal-04277487v1","host_type":""},{"url":"https://doi.org/https://doi.org/10.1242/dev.201755","host_type":""}],"fields_of_study":["Genetics, Aging, and Longevity in Model Organisms","Epigenetics and DNA Methylation","Genomics and Chromatin Dynamics","0301 basic medicine","03 medical and health sciences"],"mesh_terms":["Animals","Germ Cells","Histone Deacetylases","Histones","X Chromosome","Caenorhabditis elegans","In Situ Hybridization, Fluorescence","Caenorhabditis elegans Proteins","Sin3 Histone Deacetylase and Corepressor Complex"],"keywords":["Biology","Caenorhabditis elegans","Genetics","Histone","Germline","Gene silencing","Regulator","H3K4me3","Gene","Haploinsufficiency","Transcriptional regulation","Cell biology","Phenotype","Gene expression","Promoter","X chromosome","Proteomics","C. 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