{"doi":"10.1242/dev.199512","title":"A single-cell atlas of mouse lung development","abstract":"Lung organogenesis requires precise timing and coordination to effect spatial organization and function of the parenchymal cells. To provide a systematic broad-based view of the mechanisms governing the dynamic alterations in parenchymal cells over crucial periods of development, we performed a single-cell RNA-sequencing time-series yielding 102,571 epithelial, endothelial and mesenchymal cells across nine time points from embryonic day 12 to postnatal day 14 in mice. Combining computational fate-likelihood prediction with RNA in situ hybridization and immunofluorescence, we explore lineage relationships during the saccular to alveolar stage transition. The utility of this publicly searchable atlas resource (www.sucrelab.org/lungcells) is exemplified by discoveries of the complexity of type 1 pneumocyte function and characterization of mesenchymal Wnt expression patterns during the saccular and alveolar stages - wherein major expansion of the gas-exchange surface occurs. We provide an integrated view of cellular dynamics in epithelial, endothelial and mesenchymal cell populations during lung organogenesis.","journal":"Development","year":2021,"id":147226,"datarank":3.079871359592831,"base_score":5.135798437050262,"endowment":5.135798437050262,"self_citation_contribution":0.7703697655575394,"citation_network_contribution":2.309501594035291,"self_endowment_contribution":0.7703697655575394,"citer_contribution":2.309501594035291,"corpus_percentile":92.81349114256982,"corpus_rank":930,"citation_count":169,"citer_count":100,"citers_with_citation_signal":100,"citers_with_endowment":100,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.8526,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":264685,"name":"Erin J. Plosa","orcid":"0000-0001-7165-1722","position":1,"is_corresponding":false},{"id":264688,"name":"John T. Benjamin","orcid":"0000-0002-0930-1753","position":2,"is_corresponding":false},{"id":264684,"name":"Bryce Schuler","orcid":"0000-0001-9111-3702","position":3,"is_corresponding":false},{"id":34714,"name":"Arun C. Habermann","orcid":"0000-0002-7564-6370","position":4,"is_corresponding":false},{"id":264686,"name":"Christopher S. Jetter","orcid":"0000-0002-3826-5843","position":5,"is_corresponding":false},{"id":265650,"name":"Peter M. Gulleman","orcid":null,"position":6,"is_corresponding":false},{"id":627069,"name":"Claire Bunn","orcid":null,"position":7,"is_corresponding":false},{"id":264690,"name":"Alice N. Hackett","orcid":"0000-0001-8833-7027","position":8,"is_corresponding":false},{"id":626079,"name":"Meaghan Ransom","orcid":"0000-0002-2535-4468","position":9,"is_corresponding":false},{"id":12133,"name":"Chase J. Taylor","orcid":"0000-0002-7942-0483","position":10,"is_corresponding":false},{"id":264689,"name":"David S. Nichols","orcid":"0000-0002-8066-3132","position":11,"is_corresponding":false},{"id":269507,"name":"Brittany K. Matlock","orcid":null,"position":12,"is_corresponding":false},{"id":254895,"name":"Susan H. Guttentag","orcid":"0000-0003-4420-5879","position":13,"is_corresponding":false},{"id":264692,"name":"Timothy S. Blackwell","orcid":"0000-0002-0337-7052","position":14,"is_corresponding":false},{"id":12172,"name":"Nicholas E. Banovich","orcid":"0000-0003-2604-3247","position":15,"is_corresponding":false},{"id":12177,"name":"Jonathan A. Kropski","orcid":"0000-0002-8923-1344","position":16,"is_corresponding":false},{"id":104335,"name":"Jennifer M. S. Sucre","orcid":"0000-0002-6613-1439","position":17,"is_corresponding":false},{"id":264687,"name":"Nicholas M. Negretti","orcid":"0000-0003-1022-8650","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":null,"created_at":"2026-07-18T23:42:30.206628Z","pmid":"34927678","pmcid":"PMC8722390","fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}