{"doi":"10.1242/dev.196048","title":"Androgen action in cell fate and communication during prostate development at single-cell resolution","abstract":"Androgens/androgen receptor (AR) mediated signaling pathways are essential for prostate development, morphogenesis, and regeneration. Specifically, stromal AR-signaling has been shown to be essential for prostatic initiation. However, the molecular mechanisms underlying AR-initiated mesenchymal-epithelial interactions in prostate development remain unclear. Here, using a newly generated mouse model, we directly addressed the fate and role of genetically marked AR-expressing cells during embryonic prostate development. Androgen signaling-initiated signaling pathways were identified in mesenchymal niche populations at single cell transcriptomic resolution. The dynamic cell-signaling networks regulated by stromal AR were characterized in regulating prostatic epithelial bud formation. Pseudotime analyses further revealed the differentiation trajectory and fate of AR-expressing cells in both prostatic mesenchymal and epithelial cell populations. Specifically, the cellular properties of Zeb1-expressing progenitors were assessed. Selective deletion of AR signaling in a subpopulation mesenchymal rather than epithelial cells dysregulates the expression of the master regulators and significantly impairs prostatic bud formation. These data provide novel, high-resolution evidence demonstrating the important role of mesenchymal androgen signaling as cellular niches controlling prostate early development by initiating dynamic mesenchyme-epithelia cell interactions.","journal":"Development","year":2020,"id":79099,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9521,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":411310,"name":"Adam W. Olson","orcid":"0000-0003-3118-0393","position":1,"is_corresponding":false},{"id":411311,"name":"Jinhui Wang","orcid":"0000-0002-3499-4336","position":2,"is_corresponding":false},{"id":412562,"name":"Won Kyung Kim","orcid":null,"position":3,"is_corresponding":false},{"id":411312,"name":"Jiaqi Mi","orcid":"0000-0002-6537-7795","position":4,"is_corresponding":false},{"id":297985,"name":"Hong Zeng","orcid":"0000-0002-5781-8773","position":5,"is_corresponding":false},{"id":411313,"name":"Vien Le","orcid":"0000-0003-2933-332X","position":6,"is_corresponding":false},{"id":411314,"name":"Joseph Aldahl","orcid":"0000-0001-9664-5644","position":7,"is_corresponding":false},{"id":411315,"name":"Alex Hiroto","orcid":"0000-0003-3807-9634","position":8,"is_corresponding":false},{"id":108571,"name":"Xiwei Wu","orcid":"0000-0002-7071-1671","position":9,"is_corresponding":false},{"id":411316,"name":"Zijie Sun","orcid":"0000-0002-6787-6272","position":10,"is_corresponding":false},{"id":240836,"name":"Dong Hoon Lee","orcid":"0000-0003-1329-4637","position":0,"is_corresponding":true}],"reference_count":67,"raw_metadata":null,"created_at":"2026-07-18T21:50:33.748601Z","pmid":"33318148","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}