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However, subsequent metabolomic analyses suggested that dLdh mutants compensate for the inability to produce lactate by generating excess glycerol-3-phosphate (G3P), the production of which also influences larval redox balance. Consistent with this possibility, larvae lacking both dLDH and G3P dehydrogenase (GPDH1) exhibit growth defects, synthetic lethality, and decreased glycolytic flux. Considering that human cells also generate G3P upon Lactate Dehydrogenase A (LDHA) inhibition, our findings hint at a conserved mechanism in which the coordinate regulation of lactate and G3P synthesis imparts metabolic robustness upon growing animal tissues.</jats:p>","journal":"Development","year":2019,"id":609187,"datarank":0.5775221402565088,"base_score":3.8501476017100584,"endowment":3.8501476017100584,"self_citation_contribution":0.5775221402565088,"citation_network_contribution":0.0,"self_endowment_contribution":0.5775221402565088,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":46,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":941951,"name":"Madhulika Rai","orcid":"0000-0003-0228-285X","position":1,"is_corresponding":false},{"id":410850,"name":"Kasun Buddika","orcid":"0000-0002-9872-4765","position":2,"is_corresponding":false},{"id":471764,"name":"Maria C. 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The resulting metabolic program is ideally suited to synthesize macromolecules and mimics the manner by which cancer cells rely on aerobic glycolysis. To explore the potential role of Drosophila dLDH in promoting biosynthesis, we examined how dLdh mutations influence larval development. Our studies unexpectantly found that dLdh mutants grow at a normal rate, indicating that dLDH is dispensable for larval biomass production. However, subsequent metabolomic analyses suggested that dLdh mutants compensate for the inability to produce lactate by generating excess glycerol-3-phosphate (G3P), the production of which also influences larval redox balance. Consistent with this possibility, larvae lacking both dLDH and G3P dehydrogenase (GPDH1) exhibit growth defects, synthetic lethality, and decreased glycolytic flux. Considering that human cells also generate G3P upon Lactate Dehydrogenase A (LDHA) inhibition, our findings hint at a conserved mechanism in which the coordinate regulation of lactate and G3P synthesis imparts metabolic robustness upon growing animal tissues.</jats:p>","is_dataset_classified":null,"base_score":3.8501476017100584,"endowment":3.8501476017100584,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31399469","pmcid":"PMC6765128","openalex_id":"https://openalex.org/W2968526955","authors":[],"funders":[{"funder_name":"National Institute of General Medical Sciences","grant_id":"1R35GM119557","title":null},{"funder_name":"Division of Integrative Organismal Systems","grant_id":"1149178","title":null},{"funder_name":"Division of Chemistry","grant_id":"1726633","title":null},{"funder_name":"NIH HHS","grant_id":"P40 OD010949","title":null},{"funder_name":"NHGRI NIH HHS","grant_id":"U41 HG000739","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"U54 DK110858","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM124220","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R35 GM119557","title":null},{"funder_name":"NIH HHS","grant_id":"S10 OD016232","title":null},{"funder_name":"NIH HHS","grant_id":"P40 OD018537","title":null},{"funder_name":"NIH HHS","grant_id":"S10 OD021505","title":null}],"total_grants":11,"fwci":2.587,"citation_percentile":0.89464667,"influential_citations":0,"citation_trend":[{"year":2019,"count":1},{"year":2020,"count":4},{"year":2021,"count":4},{"year":2022,"count":8},{"year":2023,"count":9},{"year":2024,"count":8},{"year":2025,"count":6},{"year":2026,"count":6}],"oa_status":"bronze","license":"http://www.biologists.com/user-licence-1-1/","oa_locations":[{"url":"https://dev.biologists.org/content/develop/146/17/dev175315.full.pdf","host_type":"journal"},{"url":"https://dev.biologists.org/content/develop/146/17/dev175315.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1242/dev.175315","host_type":"publisher"},{"url":"http://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.175315/1967616/dev175315.pdf","host_type":"publisher"},{"url":"http://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.175315/2063951/dev_175315v1.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/dev.175315","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/31399469","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6765128","host_type":"repository"},{"url":"http://iu.tind.io/record/2349","host_type":"repository"},{"url":"http://dev.biologists.org/cgi/content/short/146/17/dev175315","host_type":"repository"},{"url":"https://hdl.handle.net/2022/27118","host_type":"repository"}],"fields_of_study":["Physiological and biochemical adaptations","Neurobiology and Insect Physiology Research","Genetics, Aging, and Longevity in Model Organisms"],"mesh_terms":["Sugars","Adenosine Triphosphate","Animals","Drosophila melanogaster","Female","Glycerolphosphate Dehydrogenase","Glycolysis","Homeostasis","L-Lactate Dehydrogenase","Larva","Male","Mutation","NAD","Oxidation-Reduction","Lactic Acid","Animals, Genetically Modified"],"keywords":["Biology","Lactate dehydrogenase","Glycolysis","Dehydrogenase","Biochemistry","Metabolism","Drosophila melanogaster","Carbohydrate metabolism","Cell biology","Enzyme","Gene","Drosophila","Redox balance","glycerol-3-phosphate dehydrogenase","Aerobic Glycolysis"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"},{"name":"uniprot"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-31T04:15:16.666945Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}