{"doi":"10.1242/dev.120.9.2651","title":"Stage-specific requirement for myosin II during <i>Dictyostelium</i> development","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>Dictyostelium cells that lack a functional myosin II heavy chain are motile and are capable of aggregation, but fail to undergo further multicellular development. We have used a Dictyostelium mutant expressing a cold-sensitive myosin heavy chain to examine the requirement for myosin throughout the course of development. The loss of myosin function upon cooling is rapid and reversible. Tempera-ture-shift experiments reveal that myosin is essential during two different stages of development. During aggregation, myosin function appears to be necessary for cells to sort correctly in a way that allows further development to occur. During the final stage of development, it is required for the formation of a complete stalk and the raising of the spore head. Development between those stages, however, proceeds normally in the absence of myosin function. Aggregates at non-permissive temperature undergo an aberrant form of development resulting in a ball of cells. Calcofluor staining and reporter gene fusions reveal that these structures contain defective spores and a miniature stalk.</jats:p>","journal":"Development","year":1994,"id":678677,"datarank":0.5709993734655481,"base_score":3.8066624897703196,"endowment":3.8066624897703196,"self_citation_contribution":0.5709993734655481,"citation_network_contribution":0.0,"self_endowment_contribution":0.5709993734655481,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":44,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1773205,"name":"B. Patterson","orcid":null,"position":1,"is_corresponding":false},{"id":195565,"name":"J. A. Spudich","orcid":null,"position":2,"is_corresponding":false},{"id":1773204,"name":"M. L. Springer","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Stage-specific requirement for myosin II during <i>Dictyostelium</i> development","abstract":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>Dictyostelium cells that lack a functional myosin II heavy chain are motile and are capable of aggregation, but fail to undergo further multicellular development. We have used a Dictyostelium mutant expressing a cold-sensitive myosin heavy chain to examine the requirement for myosin throughout the course of development. The loss of myosin function upon cooling is rapid and reversible. Tempera-ture-shift experiments reveal that myosin is essential during two different stages of development. During aggregation, myosin function appears to be necessary for cells to sort correctly in a way that allows further development to occur. During the final stage of development, it is required for the formation of a complete stalk and the raising of the spore head. Development between those stages, however, proceeds normally in the absence of myosin function. Aggregates at non-permissive temperature undergo an aberrant form of development resulting in a ball of cells. Calcofluor staining and reporter gene fusions reveal that these structures contain defective spores and a miniature stalk.</jats:p>","is_dataset_classified":null,"base_score":3.8066624897703196,"endowment":3.8066624897703196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7956839","pmcid":null,"openalex_id":"https://openalex.org/W1937669071","authors":[],"funders":[{"funder_name":"NIAMS NIH HHS","grant_id":"5F32AR08235-02","title":null},{"funder_name":"NCI NIH HHS","grant_id":"5T32CA09151-18","title":null},{"funder_name":"NCI NIH HHS","grant_id":"5T32CA09302-15","title":null}],"total_grants":3,"fwci":1.2678,"citation_percentile":0.79219543,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2016,"count":1},{"year":2019,"count":1}],"oa_status":"closed","license":"http://www.biologists.com/user-licence-1-1/","oa_locations":[{"url":"https://journals.biologists.com/dev/article-pdf/120/9/2651/3595118/develop_120_9_2651.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1242/dev.120.9.2651","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7956839","host_type":"repository"}],"fields_of_study":["Cellular Mechanics and Interactions","Cardiomyopathy and Myosin Studies","Muscle Physiology and Disorders","Animals","Cell Aggregation","Cold Temperature","Dictyostelium","Genes, Protozoan","Mutation","Myosins","Phenotype"],"mesh_terms":["Animals","Cell Aggregation","Cold Temperature","Dictyostelium","Mutation","Myosins","Phenotype","Genes, Protozoan"],"keywords":["Myosin","Dictyostelium","Biology","Cell biology","Multicellular organism","Mycetozoa","Mutant","Dictyostelium discoideum","Actin","Biochemistry","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T05:47:55.138018Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}