{"doi":"10.1210/jendso/bvab088","title":"Insulin Infusion Is Linked to Increased <i>NPPC</i> Expression in Muscle and Plasma C-type Natriuretic Peptide in Male Dogs","abstract":"Abstract The purpose of this study was to assess insulin-stimulated gene expression in canine skeletal muscle with a particular focus on NPPC, the gene that encodes C-type natriuretic peptide, a key hormonal regulator of cardiometabolic function. Four conscious canines underwent hyperinsulinemic, euglycemic clamp studies. Skeletal muscle biopsy and arterial plasma samples were collected under basal and insulin-stimulated conditions. Bulk RNA sequencing of muscle tissue was performed to identify differentially expressed genes between these 2 steady-state conditions. Our results showed that NPPC was the most highly expressed gene in skeletal muscle in response to insulin infusion, rising 4-fold between basal and insulin-stimulated conditions. In support of our RNA sequencing data, we found that raising the plasma insulin concentration 15-fold above basal elicited a 2-fold (P = 0.0001) increase in arterial plasma concentrations of N-terminal prohormone C-type natriuretic peptide. Our data suggest that insulin may play a role in stimulating secretion of C-type natriuretic peptide by skeletal muscle. In this context, C-type natriuretic peptide may act in a paracrine manner to facilitate muscle–vascular bed crosstalk and potentiate insulin-mediated vasodilation. This could serve to enhance insulin and glucose delivery, particularly in the postprandial absorptive state.","journal":"Journal of the Endocrine Society","year":2021,"id":206690,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9626,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":779314,"name":"Guillaume Kraft","orcid":"0000-0001-8538-4725","position":1,"is_corresponding":false},{"id":779315,"name":"Ben Farmer","orcid":"0000-0002-2587-0008","position":2,"is_corresponding":false},{"id":779821,"name":"Marta S. Smith","orcid":null,"position":3,"is_corresponding":false},{"id":791629,"name":"David C. LaNeve","orcid":null,"position":4,"is_corresponding":false},{"id":791630,"name":"Phillip E. Williams","orcid":null,"position":5,"is_corresponding":false},{"id":727290,"name":"Kelsey Tomasek","orcid":"0009-0007-6576-8933","position":6,"is_corresponding":false},{"id":415538,"name":"Yan Ru Su","orcid":"0000-0001-7733-1041","position":7,"is_corresponding":false},{"id":791122,"name":"Christopher Wilson","orcid":"0000-0002-7562-8420","position":8,"is_corresponding":false},{"id":791123,"name":"Mark Thompson","orcid":"0000-0001-8986-3088","position":9,"is_corresponding":false},{"id":779316,"name":"Alan D. Cherrington","orcid":"0000-0001-8927-2560","position":10,"is_corresponding":false},{"id":250516,"name":"Katie C. Coate","orcid":"0000-0002-6604-3906","position":11,"is_corresponding":false},{"id":237695,"name":"Justin M. Gregory","orcid":"0000-0003-2700-0062","position":0,"is_corresponding":true}],"reference_count":34,"raw_metadata":null,"created_at":"2026-07-18T23:51:41.571653Z","pmid":"34131611","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}