{"doi":"10.1210/en.2007-1396","title":"β1-Adrenoceptor Expression in Rat Anterior Pituitary Gonadotrophs and in Mouse αT3-1 and LβT2 Gonadotrophic Cell Lines","abstract":"<jats:p>The rat anterior pituitary expresses β2-adrenoceptors (ARs) on somatotrophs, lactotrophs, and corticotrophs. The present study investigates whether β1-ARs exist in the anterior pituitary, in which cell type(s) they are found, and whether they are regulated by glucocorticoids. As determined by quantitative RT-PCR and Western immunoblotting, the rat anterior pituitary expressed β1-AR mRNA and protein. Unlike the β2-AR, expression decreased to very low levels after 5-d aggregate cell culture but was strongly up-regulated in a dose- and time-dependent manner by dexamethasone (DEX). Glucocorticoids attenuated isoproterenol-induced down-regulation of β1-AR mRNA levels. As examined by immunofluorescence confocal microscopy, β1-AR immunoreactivity was detected in a subpopulation of gonadotrophs, but not in somatotrophs, lactotrophs, corticotrophs, thyrotrophs, or folliculo-stellate cells. β1-AR-immunoreactivity cells were often surrounded by cup-shaped lactotrophs. Consistent with these findings, β1-AR mRNA was considerably more abundant in the gonadotrophic αT3-1 and LβT2 cell lines than in the GHFT, GH3, and TtT/GF cell lines. DEX did not affect expression level in the cell lines. DEX also failed to up-regulate β1-AR mRNA levels in aggregates from a subpopulation enriched in large gonadotrophs obtained by gradient sedimentation. In contrast, excessive DEX-dependent up-regulation of β1-AR mRNA was found in a subpopulation enriched in small nonhormonal cells. The present data indicate that β1-AR is expressed in a subpopulation of gonadotrophs with a topographical relationship to lactotrophs. However, the glucocorticoid-induced up-regulation does not seem to occur directly in the gonadotrophs but within (an)other unidentified cell type(s), or is transduced by that cell type on gonadotrophs.</jats:p>","journal":"Endocrinology","year":2008,"id":36502,"datarank":0.43156497850566056,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.1396784561473635,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.1396784561473635,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":5,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":184722,"name":"Olga Krylyshkina","orcid":null,"position":1,"is_corresponding":false},{"id":184723,"name":"Nicole Hersmus","orcid":null,"position":2,"is_corresponding":false},{"id":184724,"name":"Hugo Vankelecom","orcid":null,"position":3,"is_corresponding":false},{"id":154773,"name":"Carl Denef","orcid":null,"position":4,"is_corresponding":false},{"id":184721,"name":"Kristel Janssens","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18202120","pmcid":null,"openalex_id":"https://openalex.org/W2012470696","authors":[],"funders":[],"total_grants":0,"fwci":0.4408,"citation_percentile":0.57330827,"influential_citations":1,"citation_trend":[{"year":2019,"count":1},{"year":2025,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://academic.oup.com/endo/article-pdf/149/5/2313/9000321/endo2313.pdf","host_type":"BRONZE"},{"url":"http://academic.oup.com/endo/article-pdf/149/5/2313/9000321/endo2313.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1210/en.2007-1396","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18202120","host_type":"repository"},{"url":"https://lirias.kuleuven.be/bitstream/123456789/242375/3/Janssens-Endocrinology%202008.pdf","host_type":"repository"}],"fields_of_study":["Stress Responses and Cortisol","Receptor Mechanisms and Signaling","Adipose Tissue and Metabolism","Biology","Medicine","Adrenergic beta-1 Receptor Agonists","Adrenergic beta-2 Receptor Agonists","Animals","Cell Line","Dexamethasone","Gene Expression Regulation","Glucocorticoids","Glycoprotein Hormones, alpha Subunit","Gonadotrophs","Isoproterenol","Luteinizing Hormone, beta Subunit","Male","Mice","Pituitary Gland, Anterior","Pituitary Gland, Intermediate","RNA, Messenger","Rats","Rats, Wistar","Receptors, Adrenergic, beta-1","Receptors, Adrenergic, beta-2"],"mesh_terms":["Animals","Cell Line","Dexamethasone","Gene Expression Regulation","Glucocorticoids","Isoproterenol","Male","Pituitary Gland, Anterior","RNA, Messenger","Glycoprotein Hormones, alpha Subunit","Rats, Wistar","Receptors, Adrenergic, beta-1","Receptors, Adrenergic, beta-2","Luteinizing Hormone, beta Subunit","Mice","Rats","Gonadotrophs","Pituitary Gland, Intermediate","Adrenergic beta-1 Receptor Agonists","Adrenergic beta-2 Receptor Agonists"],"keywords":["Prolactin cell","Gonadotropic cell","Internal medicine","Endocrinology","Corticotropic cell","Somatotropic cell","Anterior pituitary","Thyrotropic cell","Biology","Pituitary gland","Hormone","Medicine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T16:11:17.860020Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}