{"doi":"10.1201/9781003298038-37","title":"Chemical Tools for Decoding Redox Signaling at the Host–Microbe Interface","abstract":"Host cells deploy a variety of chemically reactive small molecules, such as reactive oxygen species and reactive nitrogen species, to defend themselves against invading pathogens. These molecules are primarily known for their bactericidal activity; however, oxidants like hydrogen peroxide and nitric oxide can also function as signaling molecules that posttranslationally modify redoxsensitive amino acids under non-pathological conditions. Chemical proteomic methods rely on small-molecule, activity-based probes to facilitate the detection of reactive amino acids within a whole proteome. The indirect detection of oxidized cysteines using isoTOP-ABPP or OxICAT offers an unbiased approach to identifying OxiPTMs. Chemical profiling technologies coupled with chemotype-specific probes should help establish the contribution of specific OxiPTMs to host-microbe interactions. A recent quantitative characterization of the mouse cysteine redox proteome demonstrates the feasibility of mapping tissue-specific oxidation events in vivo. Harnessing these technologies to detect oxidative crosstalk at the host-microbe interface could uncover a vast network of ROS-mediated signaling pathways that shape bacterial and host physiology.","journal":null,"year":2023,"id":411160,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9517,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":432764,"name":"Stavroula K. Hatzios","orcid":"0000-0001-8658-8949","position":1,"is_corresponding":false},{"id":433384,"name":"Elizabeth M. Gordon","orcid":null,"position":0,"is_corresponding":true}],"reference_count":21,"raw_metadata":null,"created_at":"2026-07-19T01:21:38.940853Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}