{"doi":"10.1200/jco.2016.34.15_suppl.e17085","title":"Gene expression profiling of single circulating tumor cells in ovarian cancer: Establishment of a multi-marker gene panel.","abstract":null,"journal":"Journal of Clinical Oncology","year":2016,"id":639613,"datarank":0.19040105554756587,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.02560921224734942,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.02560921224734942,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":2,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1661879,"name":"Christina Blassl","orcid":null,"position":1,"is_corresponding":false},{"id":1661880,"name":"Jan Dominik Kuhlmann","orcid":null,"position":2,"is_corresponding":false},{"id":1661881,"name":"Alessandra Webers","orcid":null,"position":3,"is_corresponding":false},{"id":850771,"name":"Pauline Wimberger","orcid":"0000-0002-7380-577X","position":4,"is_corresponding":false},{"id":1541943,"name":"Hans Neubauer","orcid":null,"position":5,"is_corresponding":false},{"id":842787,"name":"Tanja N. Fehm","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Gene expression profiling of single circulating tumor cells in ovarian cancer: Establishment of a multi-marker gene panel.","abstract":"e17085 Background: Circulating tumor cells (CTCs) in the blood of ovarian cancer patients (OCP) are associated with decreased overall survival, whereby CTCs with epithelial-mesenchymal-transition (EMT) or stem-like traits are supposed to be involved in metastatic progression and recurrence. Investigating the transcriptional profiles of CTCs might help to identify therapy resistant tumor cells and to overcome treatment failure. We established a multi-marker PCR for the molecular characterization of single CTCs, detecting epithelial (EpCAM, Muc1, CK5/7), EMT (N-cad, vim, Snai1/2, CD117, CD146, CD49f) and stem cell (CD44, ALDH1A1, Nanog, SOX2, Notch1/4, Oct4, Lin28) associated transcripts. Methods: Primer specificity and PCR-performance was successfully validated on OvCar3 genomic DNA and cDNA. Assay sensitivity was evaluated by spiking experiments and immunomagnetic tumor cell enrichment (AdnaTest OvarianCancerSelect), resulting in a 100% concordance for the epithelial markers EpCAM and Muc-1 to the AdnaTest OvarianCancerDetect. By processing blood from OCP, high assay sensitivity could be verified. In blood of healthy donors no signals for epithelial markers were detected, for EMT and stem cell markers, however, signals were obtained mainly originating from leukocytes which calls for single cell analysis. Results: By using Ovcar3 cells we successfully established a workflow enabling the characterization of single CTCs. It comprises CTC enrichment, single cell isolation via micromanipulation, reverse transcription and multiplex-PCR analysis. Finally, 15 single CTCs from 3 OCP were analyzed and found to be positive for stem cell and EMT markers. Albeit, inter-cellular and intra/inter-patient heterogeneity and co-expression of epithelial, mesenchymal and stem cell transcripts on the same CTC was observed. Conclusions: We have established a robust workflow to perform sensitive single cell panel gene expression analysis without the need of pre-amplification steps. Our data point towards a heterogeneous expression of stem cell and EMT associated transcripts in ovarian cancer CTCs and might help to identify therapy resistant CTCs to predict treatment response.","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19767382","pmcid":null,"openalex_id":"https://openalex.org/W2891723653","authors":[],"funders":[{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"unidentified","title":"unidentified"}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2021,"count":1},{"year":2024,"count":1}],"oa_status":"closed","license":"Wiley Online Library User Agreement","oa_locations":[{"url":"http://ascopubs.org/doi/pdfdirect/10.1200/JCO.2016.34.15_suppl.e17085","host_type":"publisher"},{"url":"https://doi.org/10.1200/jco.2016.34.15_suppl.e17085","host_type":"journal"},{"url":"https://doi.org/10.1016/j.molonc.2016.04.002","host_type":""},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdfdirect/10.1016/j.molonc.2016.04.002","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/27157930","host_type":""},{"url":"https://doaj.org/article/39badcdde21c40768ca529d8feac4a1a","host_type":""},{"url":"https://dx.doi.org/10.1200/jco.2016.34.15_suppl.e17085","host_type":""},{"url":"https://dx.doi.org/10.1016/j.molonc.2016.04.002","host_type":""}],"fields_of_study":["Cancer Cells and Metastasis","Cancer Genomics and Diagnostics","Cancer-related molecular mechanisms research","03 medical and health sciences","0302 clinical medicine"],"mesh_terms":[],"keywords":["Circulating tumor cell","Epithelial cell adhesion molecule","Stem cell marker","CD44","Cancer research","SOX2","Cancer stem cell","Stem cell","Homeobox protein NANOG","Ovarian cancer","Mesenchymal stem cell","Single-cell analysis","Biology","Epithelial–mesenchymal transition","Medicine","Cancer","Molecular biology","Cell","Pathology","Metastasis","Gene","Internal medicine","Induced pluripotent stem cell","Embryonic stem cell","Ovarian Neoplasms","Epithelial-Mesenchymal Transition","Gene Expression Profiling","Circulating tumor cells","Neoplasms. Tumors. Oncology. Including cancer and carcinogens","Reproducibility of Results","Epithelial Cells","Cell Separation","Multiplex‐RT‐PCR","Neoplastic Cells, Circulating","Real-Time Polymerase Chain Reaction","Gene Expression Regulation, Neoplastic","Single cell expression analysis","Cell Line, Tumor","Biomarkers, Tumor","Humans","Female","RC254-282"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"},{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T01:01:06.627694Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}