{"doi":"10.1194/jlr.m023119","title":"HIV-1 Nef mobilizes lipid rafts in macrophages through a pathway that competes with ABCA1-dependent cholesterol efflux","abstract":null,"journal":"Journal of Lipid Research","year":2012,"id":682051,"datarank":0.6535063240034389,"base_score":4.356708826689592,"endowment":4.356708826689592,"self_citation_contribution":0.6535063240034389,"citation_network_contribution":0.0,"self_endowment_contribution":0.6535063240034389,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":77,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1781904,"name":"Angela Grant","orcid":null,"position":1,"is_corresponding":false},{"id":264534,"name":"Nigora Mukhamedova","orcid":"0000-0002-5924-1816","position":2,"is_corresponding":false},{"id":477898,"name":"Tatiana Pushkarsky","orcid":null,"position":3,"is_corresponding":false},{"id":1781905,"name":"Lucas Jennelle","orcid":null,"position":4,"is_corresponding":false},{"id":343439,"name":"Larisa Dubrovsky","orcid":null,"position":5,"is_corresponding":false},{"id":525808,"name":"Katharina Gaus","orcid":"0000-0002-8009-9658","position":6,"is_corresponding":false},{"id":708601,"name":"Michael L. Fitzgerald","orcid":"0000-0003-2346-9250","position":7,"is_corresponding":false},{"id":1374734,"name":"Dmitri Sviridov","orcid":"0000-0002-5595-0455","position":8,"is_corresponding":false},{"id":276950,"name":"Michael Bukrinsky","orcid":"0000-0002-8593-0770","position":9,"is_corresponding":false},{"id":1781903,"name":"Huanhuan L. Cui","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"HIV-1 Nef mobilizes lipid rafts in macrophages through a pathway that competes with ABCA1-dependent cholesterol efflux","abstract":"HIV infection, through the actions of viral accessory protein Nef, impairs activity of cholesterol transporter ABCA1, inhibiting cholesterol efflux from macrophages and elevating the risk of atherosclerosis. Nef also induces lipid raft formation. In this study, we demonstrate that these activities are tightly linked and affect macrophage function and HIV replication. Nef stimulated lipid raft formation in macrophage cell line RAW 264.7, and lipid rafts were also mobilized in HIV-1-infected human monocyte-derived macrophages. Nef-mediated transfer of cholesterol to lipid rafts competed with the ABCA1-dependent pathway of cholesterol efflux, and pharmacological inhibition of ABCA1 functionality or suppression of ABCA1 expression by RNAi increased Nef-dependent delivery of cholesterol to lipid rafts. Nef reduced cell-surface accessibility of ABCA1 and induced ABCA1 catabolism via the lysosomal pathway. Despite increasing the abundance of lipid rafts, expression of Nef impaired phagocytic functions of macrophages. The infectivity of the virus produced in natural target cells of HIV-1 negatively correlated with the level of ABCA1. These findings demonstrate that Nef-dependent inhibition of ABCA1 is an essential component of the viral replication strategy and underscore the role of ABCA1 as an innate anti-HIV factor.","is_dataset_classified":null,"base_score":4.356708826689592,"endowment":4.356708826689592,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22262807","pmcid":"PMC3307646","openalex_id":"https://openalex.org/W2088368059","authors":[],"funders":[{"funder_name":"NHLBI NIH HHS","grant_id":"HL-101274","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"P30 AI087714","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"R01 HL112661","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI-078743","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"1P30AI-087714-01","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"R01 HL093818","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"HL-093818","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R21 AI078743","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"R01 HL101274","title":null}],"total_grants":9,"fwci":3.115,"citation_percentile":0.9208278,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":5},{"year":2014,"count":12},{"year":2015,"count":6},{"year":2016,"count":8},{"year":2017,"count":2},{"year":2018,"count":6},{"year":2019,"count":5},{"year":2020,"count":3},{"year":2021,"count":10},{"year":2022,"count":3},{"year":2023,"count":3},{"year":2024,"count":4},{"year":2025,"count":5},{"year":2026,"count":3}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.jlr.org/content/53/4/696.full.pdf","host_type":"journal"},{"url":"http://www.jlr.org/content/53/4/696.full.pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0022227520405632?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0022227520405632?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1194/jlr.M023119","host_type":"publisher"},{"url":"https://doi.org/10.1194/jlr.m023119","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22262807","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3307646","host_type":"repository"},{"url":"https://doaj.org/article/e59aa69992e94a1c893ec568bab6cedd","host_type":"repository"}],"fields_of_study":["HIV Research and Treatment","Cholesterol and Lipid Metabolism","HIV-related health complications and treatments","ATP Binding Cassette Transporter 1","ATP-Binding Cassette Transporters","Animals","Biological Transport","Calcium Chloride","Chloroquine","Cholesterol","HIV Infections","HIV-1","HeLa Cells","Humans","Hydrocarbons, Fluorinated","Lysosomes","Macrophages","Membrane Microdomains","Mice","Protein Stability","Proteolysis","RNA Interference","Sulfonamides","Transfection","Virus Replication","nef Gene Products, Human Immunodeficiency Virus","Fluorocarbons","Benzenesulfonamides"],"mesh_terms":["Benzenesulfonamides","Animals","Biological Transport","Calcium Chloride","Chloroquine","Cholesterol","Fluorocarbons","HeLa Cells","Humans","Hydrocarbons, Fluorinated","Lysosomes","Macrophages","Sulfonamides","Transfection","Virus Replication","HIV-1","HIV Infections","ATP-Binding Cassette Transporters","Membrane Microdomains","RNA Interference","Mice","nef Gene Products, Human Immunodeficiency Virus","Protein Stability","Proteolysis","ATP Binding Cassette Transporter 1","Hela Cells"],"keywords":["ABCA1","Lipid raft","Cell biology","Efflux","Cholesterol","Biology","Macrophage","ATP Binding Cassette Transporter 1","Transfection","Chemistry","Cell culture","Biochemistry","Transporter","In vitro","Genetics","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T19:20:06.450294Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}