{"doi":"10.1186/s13059-014-0550-8","title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","abstract":"In comparative high-throughput sequencing assays, a fundamental task is the analysis of count data, such as read counts per gene in RNA-seq, for evidence of systematic changes across experimental conditions. Small replicate numbers, discreteness, large dynamic range and the presence of outliers require a suitable statistical approach. We present DESeq2, a method for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates. This enables a more quantitative analysis focused on the strength rather than the mere presence of differential expression. The DESeq2 package is available at http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html webcite.","journal":"Genome Biology","year":2014,"id":4709,"datarank":22.38335855471835,"base_score":11.48347605674494,"endowment":11.48347605674494,"self_citation_contribution":1.7225214085117413,"citation_network_contribution":20.660837146206607,"self_endowment_contribution":1.7225214085117413,"citer_contribution":20.660837146206607,"corpus_percentile":99.5,"corpus_rank":15,"citation_count":97097,"citer_count":191,"citers_with_citation_signal":191,"citers_with_endowment":191,"datacite_reuse_total":0,"is_dataset":false,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2014-12-05","authors":[{"id":37159,"name":"Keegan Korthauer","orcid":"0000-0002-4565-1654","position":1,"is_corresponding":false},{"id":41033,"name":"Simon Anders","orcid":"0000-0003-4868-1805","position":2,"is_corresponding":false},{"id":29945,"name":"Michael I. Love","orcid":"0000-0001-8401-0545","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}