{"doi":"10.1186/s12985-024-02423-w","title":"Genetic diversity of astroviruses detected in wild aquatic birds in Hong Kong","abstract":"Wild waterfowl serve as a reservoir of some astroviruses. Fecal samples from wild waterfowl collected at Hong Kong's Marshes were tested using pan-astrovirus reverse transcription-PCR. Positive samples underwent subsequent host identification using DNA barcoding. Based on deduced partial sequences, noteworthy samples from three astrovirus groups (mammalian, avian and unclassified astroviruses) were further analyzed by next-generation sequencing. One sample of Avastrovirus 4 clade, MP22-196, had a nearly complete genome identified. The results of ORF2 phylogenetic analysis and genetic distance analysis indicate that Avastrovirus 4 is classified as a distinct subclade within Avastrovirus. MP22-196 has typical astrovirus genome characteristics. The unique characteristics and potential differences of this genome, compared to other avian astrovirus sequences, involve the identification of a modified sgRNA sequence situated near the ORF2 start codon, which precedes the ORF1b stop codon. Additionally, the 3' UTR of MP22-196 is shorter than other avian astroviruses. This study expands our understanding of the Avastrovirus 4 clade.","journal":"Virology Journal","year":2024,"id":471369,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9541,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":773043,"name":"Wanying Sun","orcid":null,"position":1,"is_corresponding":false},{"id":248810,"name":"Thomas H. C. Sit","orcid":null,"position":2,"is_corresponding":false},{"id":247335,"name":"Christopher J. Brackman","orcid":"0000-0002-0108-5232","position":3,"is_corresponding":false},{"id":884739,"name":"Anne C. N. Tse","orcid":"0000-0002-6645-4369","position":4,"is_corresponding":false},{"id":1306244,"name":"Christine H. T. Bui","orcid":null,"position":5,"is_corresponding":false},{"id":1306245,"name":"Amy WY Tang","orcid":null,"position":6,"is_corresponding":false},{"id":1306246,"name":"Andrew N. C. Wong","orcid":null,"position":7,"is_corresponding":false},{"id":1306247,"name":"Andrew T. L. Tsang","orcid":null,"position":8,"is_corresponding":false},{"id":843396,"name":"Joe C T Koo","orcid":null,"position":9,"is_corresponding":false},{"id":106025,"name":"Samuel M. S. Cheng","orcid":"0000-0001-7293-2331","position":10,"is_corresponding":false},{"id":7188,"name":"Malik Peiris","orcid":"0000-0001-8217-5995","position":11,"is_corresponding":false},{"id":104946,"name":"Alex W. H. Chin","orcid":"0000-0002-6556-9092","position":12,"is_corresponding":false},{"id":77539,"name":"Leo L. M. Poon","orcid":"0000-0002-9101-7953","position":13,"is_corresponding":false},{"id":106028,"name":"Daisy Y.M. Ng","orcid":"0009-0000-5926-1043","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:05:44.736405Z","pmid":"38972989","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}