{"doi":"10.1186/s12964-025-02563-8","title":"Donor-dependent heterogeneity in therapeutic effects of adipose tissue extracellular vesicles","abstract":"BACKGROUND: Extracellular vesicles (EVs) are promising therapeutics for diseases associated with inflammation and tissue remodeling. However, a major limitation for the clinical translation of EV therapeutics is heterogeneity, which is donor dependent. In this study we sought to assess the physiochemical characteristics and therapeutic efficacy of tissue-derived EVs from different donors. Donor-dependent therapeutic effects of cell culture and biofluid-derived EVs have previously been shown, but remains largely unknown for tissue-derived EVs. We obtained EV-enriched samples from various sources of adipose tissue and examined their effect in reducing inflammation in a highly translational model of myocarditis. RESULTS: We demonstrate that the molecular composition of EVs varies depending on the donor and that therapeutic efficiency is donor-dependent even when controlling for age and sex. CONCLUSIONS: Our findings indicate that further research is needed to identify critical donor characteristics that predict therapeutic ability of individual or pooled adipose tissue-derived EVs to reduce inflammation and fibrosis.","journal":"Cell Communication and Signaling","year":2025,"id":548928,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9584,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1069362,"name":"Presley Giresi","orcid":"0000-0002-0095-6198","position":1,"is_corresponding":false},{"id":1069361,"name":"Vivian Xu","orcid":"0000-0002-6649-9081","position":2,"is_corresponding":false},{"id":1201716,"name":"Elizabeth J McCabe","orcid":"0000-0001-6711-4051","position":3,"is_corresponding":false},{"id":1229655,"name":"Jessica J. Fliess","orcid":"0009-0004-7689-3172","position":4,"is_corresponding":false},{"id":1443073,"name":"Axel Puls","orcid":null,"position":5,"is_corresponding":false},{"id":970198,"name":"Matthew E. Auda","orcid":"0000-0002-3679-5365","position":6,"is_corresponding":false},{"id":1069360,"name":"Molly M. Watkins","orcid":"0000-0001-9657-3142","position":7,"is_corresponding":false},{"id":275011,"name":"Sierra A. Walker","orcid":"0000-0002-9201-0833","position":8,"is_corresponding":false},{"id":248039,"name":"Damian N. Di Florio","orcid":"0000-0003-1619-5394","position":9,"is_corresponding":false},{"id":678098,"name":"Dalila Iannotta","orcid":"0000-0002-9189-4382","position":10,"is_corresponding":false},{"id":734052,"name":"Emily R. Whelan","orcid":"0000-0002-0410-0549","position":11,"is_corresponding":false},{"id":734506,"name":"Logan P. Macomb","orcid":null,"position":12,"is_corresponding":false},{"id":1069363,"name":"Kevin C. Keegan","orcid":"0000-0003-2911-4725","position":13,"is_corresponding":false},{"id":666956,"name":"Angita Jain","orcid":"0000-0003-1391-8139","position":14,"is_corresponding":false},{"id":1142423,"name":"Varsini Balamurugan","orcid":null,"position":15,"is_corresponding":false},{"id":902718,"name":"Sami Khatib","orcid":"0000-0002-4458-471X","position":16,"is_corresponding":false},{"id":1037022,"name":"G. Weigel","orcid":"0009-0009-7410-923X","position":17,"is_corresponding":false},{"id":1242158,"name":"David J. Gorelov","orcid":null,"position":18,"is_corresponding":false},{"id":275013,"name":"Anthony Pham","orcid":"0000-0002-5496-0301","position":19,"is_corresponding":false},{"id":495956,"name":"Brandy Edenfield","orcid":"0009-0005-5571-9500","position":20,"is_corresponding":false},{"id":23020,"name":"Leslie T. Cooper","orcid":"0000-0003-1002-3313","position":21,"is_corresponding":false},{"id":1069364,"name":"Houssam Farres","orcid":"0000-0002-5740-1601","position":22,"is_corresponding":false},{"id":867165,"name":"Shane A. Shapiro","orcid":"0000-0001-5753-0625","position":23,"is_corresponding":false},{"id":40577,"name":"Katelyn A. Bruno","orcid":"0000-0001-8980-6059","position":24,"is_corresponding":false},{"id":233169,"name":"Joy Wolfram","orcid":"0000-0003-0579-9897","position":25,"is_corresponding":false},{"id":248042,"name":"DeLisa Fairweather","orcid":"0000-0003-3093-1810","position":26,"is_corresponding":false},{"id":850762,"name":"Danielle J. Beetler","orcid":"0000-0003-1767-5701","position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":null,"created_at":"2026-07-19T02:54:03.053965Z","pmid":"41299530","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}