{"doi":"10.1186/s12964-024-01875-5","title":"Androgen blockage impairs proliferation and function of Sertoli cells via Wee1 and Lfng","abstract":null,"journal":"Cell Communication and Signaling","year":2024,"id":642933,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1672620,"name":"Hairui Tian","orcid":null,"position":1,"is_corresponding":false},{"id":1672621,"name":"Xuemei Liang","orcid":null,"position":2,"is_corresponding":false},{"id":1672622,"name":"Yunqiang Wu","orcid":null,"position":3,"is_corresponding":false},{"id":1366968,"name":"Jian Wen","orcid":"0000-0002-2297-4346","position":4,"is_corresponding":false},{"id":237546,"name":"Zhipeng Liu","orcid":"0000-0001-7159-5038","position":5,"is_corresponding":false},{"id":436546,"name":"Xiaodong Zhao","orcid":"0000-0003-1984-5372","position":6,"is_corresponding":false},{"id":490891,"name":"Li Tao","orcid":"0000-0002-1874-1910","position":7,"is_corresponding":false},{"id":1369108,"name":"Kang Zou","orcid":"0000-0002-4959-1951","position":8,"is_corresponding":false},{"id":1672619,"name":"Wenhui Zhai","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Androgen blockage impairs proliferation and function of Sertoli cells via Wee1 and Lfng","abstract":"BACKGROUND: Androgens are essential hormones for testicular development and the maintenance of male fertility. Environmental factors, stress, aging, and psychological conditions can disrupt androgen production, impacting the androgen signaling pathway and consequently spermatogenesis. Within the testes, testosterone is produced by Leydig cells and acts on Sertoli cells by activating the androgen receptor (AR), which then translocates to the nucleus to function as a transcription factor. Despite clinical correlations between low testosterone levels and diminished sperm quality, the precise mechanism remains unclear. METHODS: This study explores the hypothesis that reduced androgen levels impair Sertoli cell function by disrupting AR transcriptional regulation. Using an androgen blockade model with enzalutamide, we investigated the impact of low androgen levels on AR target genes in Sertoli cells through ChIP-seq and RNA-seq assays. RESULTS: Our results reveal that androgen blockage increases AR enrichment on the promoter region of Wee1, promoting Wee1 expression, while decreasing binding to the promoter region of Lfng, inhibiting its expression. Increased WEE1 protein inhibits Sertoli cell proliferation, whereas reduced LFNG affects Notch modification, leading to decreased production of glial cell line-derived neurotrophic factor (GDNF), a key growth factor for spermatogonial stem cell self-renewal. CONCLUSIONS: These findings provide new insights into the molecular mechanisms by which low androgen levels interfere with Sertoli cell functions, offering novel perspectives for the clinical treatment of male reproductive disorders.","is_dataset_classified":null,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39407201","pmcid":"PMC11481299","openalex_id":"https://openalex.org/W4403428510","authors":[],"funders":[{"funder_name":"Central Military Commission Logistics Department Medical Bureau, Application Fundamental Research for Family Plan, General Program","grant_id":"23JS22","title":null}],"total_grants":1,"fwci":1.2155,"citation_percentile":0.80530198,"influential_citations":0,"citation_trend":[{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by-nc-nd","oa_locations":[{"url":"https://biosignaling.biomedcentral.com/counter/pdf/10.1186/s12964-024-01875-5","host_type":"journal"},{"url":"https://biosignaling.biomedcentral.com/counter/pdf/10.1186/s12964-024-01875-5","host_type":"publisher"},{"url":"https://link.springer.com/content/pdf/10.1186/s12964-024-01875-5.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.1186/s12964-024-01875-5/fulltext.html","host_type":"publisher"},{"url":"https://doi.org/10.1186/s12964-024-01875-5","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39407201","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11481299","host_type":"repository"},{"url":"https://doaj.org/article/5f650a954fcf43a9838c39d7361adff4","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11481299/pdf/12964_2024_Article_1875.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11481299","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11481299?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Sperm and Testicular Function","Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities","Reproductive biology and impacts on aquatic species","Animals","Male","Mice","Androgens","Benzamides","Cell Cycle Proteins","Cell Proliferation","Glial Cell Line-Derived Neurotrophic Factor","Nitriles","Phenylthiohydantoin","Protein-Tyrosine Kinases","Receptors, Androgen","Sertoli Cells","Glycosyltransferases"],"mesh_terms":["Androgens","Animals","Benzamides","Male","Nitriles","Phenylthiohydantoin","Protein-Tyrosine Kinases","Receptors, Androgen","Sertoli Cells","Transcription Factors","Glycosyltransferases","Homeodomain Proteins","Cell Cycle Proteins","Cell Proliferation","Glial Cell Line-Derived Neurotrophic Factor","Mice"],"keywords":["Sertoli cell","Androgen receptor","Wee1","Function (biology)","Androgen","Cell biology","Cancer research","Biology","Internal medicine","Endocrinology","Apoptosis","Medicine","Spermatogenesis","Cell cycle","Genetics","Hormone","Prostate cancer","Infertility","Microenvironment","Post-transcriptional Modification","Hypoandrogenism"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-08T10:58:17.027712Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}