{"doi":"10.1186/s12934-025-02845-3","title":"Genetic tools for engineering Zymomonas mobilis, Cereibacter sphaeroides and Novosphingobium aromaticivorans to improve production of bioenergy compounds","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>\n                    Limited genetic tools for non-model bacteria are one of the limiting factors for genetic studies. This review compiles genetic tools used for three non-model alpha-proteobacteria, such as\n                    <jats:italic>Zymomonas mobilis</jats:italic>\n                    ,\n                    <jats:italic>Cereibacter</jats:italic>\n                    (\n                    <jats:italic>Rhodobacter) sphaeroides</jats:italic>\n                    , and\n                    <jats:italic>Novosphingobium aromaticivorans</jats:italic>\n                    , which hold significant potential to produce industrially essential bioenergy compounds due to their distinctive metabolic pathways and resilience in extreme environments. Each of these strains has a unique genetic profile that enables them to efficiently carry out key reactions relevant to producing bioenergy compounds, such as converting sugars into bioenergy compounds and breaking down lignotoxins. Genetic tools can further optimize these strains for enhanced bioenergy compound production. This review explores the metabolic advantages of these organisms. It highlights the available array of genetic toolkits that can be shared among them to unlock their full potential for sustainable biofuel production.\n                  </jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical abstract</jats:bold>\n                  </jats:p>","journal":"Microbial Cell Factories","year":2025,"id":640823,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1665690,"name":"Vishwajit Kumar","orcid":null,"position":1,"is_corresponding":false},{"id":1665693,"name":"Jyotsna Misra","orcid":null,"position":2,"is_corresponding":false},{"id":1665695,"name":"Amritha K.P.","orcid":null,"position":3,"is_corresponding":false},{"id":991225,"name":"Balendra Sah","orcid":"0000-0002-0178-5335","position":4,"is_corresponding":false},{"id":1408329,"name":"Piyush Behari Lal","orcid":"0000-0002-6955-6790","position":5,"is_corresponding":false},{"id":1665689,"name":"Shikha Mishra","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Genetic tools for engineering Zymomonas mobilis, Cereibacter sphaeroides and Novosphingobium aromaticivorans to improve production of bioenergy compounds","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>\n                    Limited genetic tools for non-model bacteria are one of the limiting factors for genetic studies. This review compiles genetic tools used for three non-model alpha-proteobacteria, such as\n                    <jats:italic>Zymomonas mobilis</jats:italic>\n                    ,\n                    <jats:italic>Cereibacter</jats:italic>\n                    (\n                    <jats:italic>Rhodobacter) sphaeroides</jats:italic>\n                    , and\n                    <jats:italic>Novosphingobium aromaticivorans</jats:italic>\n                    , which hold significant potential to produce industrially essential bioenergy compounds due to their distinctive metabolic pathways and resilience in extreme environments. Each of these strains has a unique genetic profile that enables them to efficiently carry out key reactions relevant to producing bioenergy compounds, such as converting sugars into bioenergy compounds and breaking down lignotoxins. Genetic tools can further optimize these strains for enhanced bioenergy compound production. This review explores the metabolic advantages of these organisms. It highlights the available array of genetic toolkits that can be shared among them to unlock their full potential for sustainable biofuel production.\n                  </jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical abstract</jats:bold>\n                  </jats:p>","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"41239446","pmcid":"PMC12619356","openalex_id":"https://openalex.org/W4416222055","authors":[],"funders":[],"total_grants":0,"fwci":0.2669,"citation_percentile":0.5097343,"influential_citations":0,"citation_trend":[{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://microbialcellfactories.biomedcentral.com/counter/pdf/10.1186/s12934-025-02845-3","host_type":"journal"},{"url":"https://microbialcellfactories.biomedcentral.com/counter/pdf/10.1186/s12934-025-02845-3","host_type":"publisher"},{"url":"https://link.springer.com/content/pdf/10.1186/s12934-025-02845-3.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.1186/s12934-025-02845-3/fulltext.html","host_type":"publisher"},{"url":"https://doi.org/10.1186/s12934-025-02845-3","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/41239446","host_type":"repository"},{"url":"https://doaj.org/article/88326e1712314d8daaa4031ce4a435e2","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12619356","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC12619356","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC12619356?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Microbial bioremediation and biosurfactants","Biofuel production and bioconversion","Microbial Metabolic Engineering and Bioproduction","Zymomonas","Biofuels","Metabolic Engineering","Rhodobacter sphaeroides","Sphingomonadaceae","Genetic Engineering","Metabolic Networks and Pathways"],"mesh_terms":["Genetic Engineering","Rhodobacter sphaeroides","Zymomonas","Sphingomonadaceae","Metabolic Networks and Pathways","Biofuels","Metabolic Engineering"],"keywords":["Bioenergy","Zymomonas mobilis","Biofuel","Metabolic engineering","Limiting","Gene","Bacteria","Novosphingobium Aromaticivorans","Cereibacter Sphaeroides"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T14:07:56.454381Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}