{"doi":"10.1186/s12866-025-03758-2","title":"Antibiotic susceptibility and molecular characterization based on whole-genome sequencing of Staphylococcus aureus causing invasive infection in children and women living in Southwest China during 2018–2023","abstract":null,"journal":"BMC Microbiology","year":2025,"id":614464,"datarank":0.3453877639491069,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.0,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1583390,"name":"Ziyi Yan","orcid":null,"position":1,"is_corresponding":false},{"id":1583391,"name":"Fumei He","orcid":null,"position":2,"is_corresponding":false},{"id":1410700,"name":"Jie Chen","orcid":"0000-0002-6444-6218","position":3,"is_corresponding":false},{"id":1583392,"name":"Linghan Kuang","orcid":null,"position":4,"is_corresponding":false},{"id":1583393,"name":"Xingxin Liu","orcid":null,"position":5,"is_corresponding":false},{"id":1583394,"name":"Yali Cui","orcid":null,"position":6,"is_corresponding":false},{"id":1184272,"name":"Xia Wang","orcid":"0000-0002-6986-0979","position":7,"is_corresponding":false},{"id":1583395,"name":"Chenglin Miao","orcid":null,"position":8,"is_corresponding":false},{"id":530355,"name":"Hong Li","orcid":"0009-0008-9201-4157","position":9,"is_corresponding":false},{"id":1583396,"name":"Yongmei Jiang","orcid":null,"position":10,"is_corresponding":false},{"id":1064426,"name":"Li Liu","orcid":"0000-0001-6126-2635","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Antibiotic susceptibility and molecular characterization based on whole-genome sequencing of Staphylococcus aureus causing invasive infection in children and women living in Southwest China during 2018–2023","abstract":"BACKGROUND: Staphylococcus aureus is one of the most common pathogens that colonizes human skin/mucous membranes, where it causes local infection that can progress to invasive infection, resulting in high morbidity and mortality worldwide. This study aimed to investigate the antibiotic susceptibility and molecular characteristics of invasive S. aureus in children and women in Southwest China from 2018 to 2023 to provide novel insights helpful in preventing and treating S. aureus infections. METHODS: The demographic and clinical characteristics of patients with invasive S. aureus infection were collected and analyzed. Next-generation sequencing (NGS) and sequence analysis techniques were used to determine the molecular epidemiological characteristics of the S. aureus isolates, and the microdilution broth method was used for antimicrobial susceptibility testing. RESULTS: A total of 108 invasive S. aureus isolates, 29 methicillin-resistant S. aureus (MRSA) isolates and 79 methicillin- susceptible S. aureus (MSSA) isolates, were included. The isolates had the highest rate of resistance to PEN, at 91.67%, with all the MRSA isolates being resistant; the next highest resistance was to ERY and CLI, both at 65.74%. A total of 32 STs (including 8 novel STs) were detected and divided into 10 CCs. Moreover, 45 spa types were also detected. The main STs were ST22 (17.59%) and ST59 (15.74%), and the main CCs were CC59 (21.30%) and CC22 (19.44%). The most prevalent spa types were t309 and t437, both at 14.81%, and the SCCmec type could be assigned to two categories: IV (62.07%) and V (34.48%). Among the 29 MRSA isolates tested, CC59-IV-t437 (34.48%) and CC59-V-t437 (13.79%) were the main lineages, and among the 79 MSSA isolates, CC22-t309 (18.99%), CC1-t189 (10.13%), and CC5-t002 (7.60%) were the main lineages. Except for SXT, the resistance rates of the 29 MRSA isolates were greater than those of the MSSA isolates. Most isolates carried common virulence genes, among which the carriage rate of pvl reached 33.33%. CONCLUSIONS: This study provides valuable information, including the prevalence, molecular characteristics and antimicrobial resistance of S. aureus isolates that cause invasive infectious diseases in Southwest China, and the findings may advance the prevention and treatment of S. aureus infections.","is_dataset_classified":null,"base_score":2.302585092994046,"endowment":2.302585092994046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39871143","pmcid":"PMC11770987","openalex_id":"https://openalex.org/W4406850863","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"82102458","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"82300015","title":null},{"funder_name":"Northern Sichuan Medical College-level Scientific Research Development Fund Project","grant_id":"CBY21-QA02","title":null},{"funder_name":"Sichuan Science and Technology Program","grant_id":"2024NSFSC1555","title":null},{"funder_name":"Sichuan Science and Technology Program","grant_id":"2023NSFSC1699","title":null},{"funder_name":"Sichuan Science and Technology Program","grant_id":"2024NSFSC1942","title":null},{"funder_name":"Sichuan Science and Technology Program","grant_id":"2024YFFK0350","title":null},{"funder_name":"Sichuan Science and Technology Program","grant_id":"2023YFS0223","title":null},{"funder_name":"Sichuan Science and Technology Program","grant_id":"2023YFS0222","title":null},{"funder_name":"Science and Technology Program of Sichuan Province","grant_id":"2022YFS0235","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"2024YFC2707405","title":null}],"total_grants":11,"fwci":7.169,"citation_percentile":0.97440906,"influential_citations":0,"citation_trend":[{"year":2025,"count":3},{"year":2026,"count":6}],"oa_status":"gold","license":"cc-by-nc-nd","oa_locations":[{"url":"https://bmcmicrobiol.biomedcentral.com/counter/pdf/10.1186/s12866-025-03758-2","host_type":"journal"},{"url":"https://bmcmicrobiol.biomedcentral.com/counter/pdf/10.1186/s12866-025-03758-2","host_type":"publisher"},{"url":"https://link.springer.com/content/pdf/10.1186/s12866-025-03758-2.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.1186/s12866-025-03758-2/fulltext.html","host_type":"publisher"},{"url":"https://doi.org/10.1186/s12866-025-03758-2","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39871143","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11770987","host_type":"repository"},{"url":"https://doaj.org/article/0b53cb8bd31b4684833230dd15f57358","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11770987/pdf/12866_2025_Article_3758.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11770987","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11770987?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Antimicrobial Resistance in Staphylococcus","Bacterial Identification and Susceptibility Testing","Streptococcal Infections and Treatments","Humans","Female","China","Staphylococcus aureus","Anti-Bacterial Agents","Staphylococcal Infections","Child","Microbial Sensitivity Tests","Whole Genome Sequencing","Child, Preschool","Infant","Adult","Male","Adolescent","Young Adult","Methicillin-Resistant Staphylococcus aureus","High-Throughput Nucleotide Sequencing","Molecular Epidemiology","Genome, Bacterial","Middle Aged","Virulence Factors"],"mesh_terms":["Whole Genome Sequencing","Adolescent","Adult","Anti-Bacterial Agents","Child","Child, Preschool","China","Female","Humans","Infant","Male","Microbial Sensitivity Tests","Middle Aged","Staphylococcal Infections","Staphylococcus aureus","Genome, Bacterial","Molecular Epidemiology","Drug Resistance, Bacterial","Virulence Factors","Methicillin-Resistant Staphylococcus aureus","Young Adult","High-Throughput Nucleotide Sequencing"],"keywords":["Biology","Staphylococcus aureus","Antibiotics","Parasitology","Whole genome sequencing","Microbiology","Genome","Antibiotic resistance","Genetics","Gene","Bacteria","Zoology","SpA","virulence factors","Invasive Infection","Multilocus Sequence Typing","Whole-genome Sequencing"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero 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