{"doi":"10.1186/1471-2121-1-2","title":"C/EBPBeta and Elk-1 synergistically transactivate the c-fos serum response element","abstract":null,"journal":"BMC Cell Biology","year":2000,"id":683996,"datarank":0.5050943744979712,"base_score":3.367295829986474,"endowment":3.367295829986474,"self_citation_contribution":0.5050943744979712,"citation_network_contribution":0.0,"self_endowment_contribution":0.5050943744979712,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":28,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1786889,"name":"Linda M Bundy","orcid":null,"position":1,"is_corresponding":false},{"id":1248987,"name":"Linda Sealy","orcid":"0000-0001-7167-2654","position":2,"is_corresponding":false},{"id":1786888,"name":"Mary Hanlon","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"C/EBPBeta and Elk-1 synergistically transactivate the c-fos serum response element","abstract":"BACKGROUND: The serum response element (SRE) in the c-fos promoter is a convergence point for several signaling pathways that regulate induction of the c-fos gene. Many transcription factors regulate the SRE, including serum response factor (SRF), ternary complex factor (TCF), and CCAAT/enhancer binding protein-beta (C/EBPbeta). Independently, the TCFs and C/EBPbeta have been shown to interact with SRF and to respond to Ras-dependent signaling pathways that result in transactivation of the SRE. Due to these common observations, we addressed the possibility that C/EBPbeta and Elk-1 could both be necessary for Ras-stimulated transactivation of the SRE. RESULTS: In this report, we demonstrate that Elk-1 and C/EBPbeta functionally synergize in transactivation of both a Gal4 reporter plasmid in concert with Gal4-SRF and in transactivation of the SRE. Interestingly, this synergy is only observed upon activation of Ras-dependent signaling pathways. Furthermore, we show that Elk-1 and C/EBPbeta could interact both in an in vitro GST-pulldown assay and in an in vivo co-immunoprecipitation assay. The in vivo interaction between the two proteins is dependent on the presence of activated Ras. We have also shown that the C-terminal domain of C/EBPbeta and the N-terminal domain of Elk-1 are necessary for the proteins to interact. CONCLUSIONS: These data show that C/EBPbeta and Elk-1 synergize in SRF dependent transcription of both a Gal-4 reporter and the SRE. This suggests that SRF, TCF, and C/EBPbeta are all necessary for maximal induction of the c-fos SRE in response to mitogenic signaling by Ras.","is_dataset_classified":null,"base_score":3.367295829986474,"endowment":3.367295829986474,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11151091","pmcid":"PMC29063","openalex_id":"https://openalex.org/W1578840628","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"5T32GM08554","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"5T32DK07563","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"T32 DK007563","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM008554","title":null},{"funder_name":"National Institutes of Health","grant_id":"5T32GM008554-22","title":"Cellular, Biochemical and Molecular Sciences Training Program"},{"funder_name":"National Institutes of Health","grant_id":"2T32DK007563-11","title":"MULTIDISCIPLINARY TRAINING IN MOLECULAR ENDOCRINOLOGY"}],"total_grants":6,"fwci":0.7105,"citation_percentile":0.67592969,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2015,"count":1},{"year":2017,"count":2},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://bmcmolcellbiol.biomedcentral.com/counter/pdf/10.1186/1471-2121-1-2","host_type":"journal"},{"url":"https://bmcmolcellbiol.biomedcentral.com/counter/pdf/10.1186/1471-2121-1-2","host_type":"publisher"},{"url":"https://link.springer.com/content/pdf/10.1186/1471-2121-1-2.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.1186/1471-2121-1-2/fulltext.html","host_type":"publisher"},{"url":"https://doi.org/10.1186/1471-2121-1-2","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11151091","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/29063","host_type":"repository"},{"url":"https://doaj.org/article/2f00e6474eba4465842f23fa92211e82","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC29063","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC29063?pdf=render","host_type":"Europe_PMC"},{"url":"https://bmcmolcellbiol.biomedcentral.com/track/pdf/10.1186/1471-2121-1-2","host_type":""},{"url":"http://dx.doi.org/10.1186/1471-2121-1-2","host_type":""},{"url":"https://dx.doi.org/10.1186/1471-2121-1-2","host_type":""}],"fields_of_study":["Protein Kinase Regulation and GTPase Signaling","Neutrophil, Myeloperoxidase and Oxidative Mechanisms","Adenosine and Purinergic Signaling","0301 basic medicine","0303 health sciences","03 medical and health sciences","3T3 Cells","Animals","CCAAT-Enhancer-Binding Protein-beta","COS Cells","Cell Line","Chlorocebus aethiops","DNA-Binding Proteins","Mice","Peptides","Protein Interaction Mapping","Proto-Oncogene Proteins","Proto-Oncogene Proteins c-fos","Proto-Oncogene Proteins p21(ras)","Serum Response Element","Signal Transduction","Transcription Factors","ets-Domain Protein Elk-1"],"mesh_terms":["Animals","Cell Line","Chlorocebus aethiops","DNA-Binding Proteins","Peptides","Proto-Oncogene Proteins","Transcription Factors","Signal Transduction","Proto-Oncogene Proteins p21(ras)","3T3 Cells","Proto-Oncogene Proteins c-fos","COS Cells","CCAAT-Enhancer-Binding Protein-beta","Protein Interaction Mapping","Serum Response Element","ets-Domain Protein Elk-1","Mice"],"keywords":["Transactivation","Ccaat-enhancer-binding proteins","Serum Response Element","Serum response factor","Transcription factor","Biology","Molecular biology","Signal transduction","Cell biology","Reporter gene","Coactivator","DNA-binding protein","Gene expression","Gene","Biochemistry","QH573-671","CCAAT-Enhancer-Binding Protein-beta","3T3 Cells","Cell Line","DNA-Binding Proteins","Proto-Oncogene Proteins p21(ras)","Mice","Proto-Oncogene Proteins","COS Cells","Chlorocebus aethiops","Protein Interaction Mapping","Animals","Cytology","Peptides","Proto-Oncogene Proteins c-fos","Research Article","Transcription Factors","ets-Domain Protein Elk-1"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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