{"doi":"10.1182/blood.v85.5.1396.bloodjournal8551396","title":"Posttransplantation lymphoproliferative disorders frequently contain type A and not type B Epstein-Barr virus","abstract":"<jats:p>Two families of Epstein-Barr virus (EBV), type A and type B, have been defined on the basis of sequence divergence in the EBNA-2 gene. Type A EBV immortalizes B cells more efficiently in vitro and infects immunocompetent individuals more commonly than type B EBV. However, increased rates of infection by type B EBV are seen in immunocompromised hosts and in many lymphoid neoplasms associated with immunocompromise. The posttransplantation lymphoproliferative disorders (PT-LPDs) are a heterogeneous group of B-cell neoplasms that arise in the setting of immunosuppressive therapy, and are associated with EBV infection. Whether type A and/or type B EBV are associated with PT-LPDs is unknown. Therefore, we investigated 27 PT-LPD lesions from 22 solid- organ transplant recipients by polymerase chain reaction (PCR) at the EBNA-2 and EBNA-3c loci to detect sequence deletions that distinguish the two EBV families. Another locus, EBER, was examined by single- strand conformation polymorphism analysis (SSCP), in conjunction with direct sequencing in selected cases. Type A EBV was found in 24 of 27 cases (89%) as seen by amplification of the EBNA-2 and EBNA-3c regions. Four different EBER polymorphisms were detected, confirming the presence of different type A EBV isolates among these cases. Three cases were negative for infection by EBV. Surprisingly, despite the immunocompromised state of the hosts, none of the 27 PT-LPD lesions harbored type B EBV. Thus, although type B EBV may commonly infect peripheral blood lymphocytes in immunocompromised individuals, they do not appear to induce readily PT-LPD formation.</jats:p>","journal":"Blood","year":1995,"id":31324,"datarank":6.46178915943381,"base_score":4.762173934797756,"endowment":4.762173934797756,"self_citation_contribution":0.7143260902196635,"citation_network_contribution":5.747463069214146,"self_endowment_contribution":0.7143260902196635,"citer_contribution":5.747463069214146,"corpus_percentile":null,"corpus_rank":null,"citation_count":116,"citer_count":102,"citers_with_citation_signal":93,"citers_with_endowment":93,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":168124,"name":"E Cesarman","orcid":null,"position":1,"is_corresponding":false},{"id":168125,"name":"YF Liu","orcid":null,"position":2,"is_corresponding":false},{"id":168126,"name":"RE Michler","orcid":null,"position":3,"is_corresponding":false},{"id":168127,"name":"DM Knowles","orcid":null,"position":4,"is_corresponding":false},{"id":168123,"name":"D Frank","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.762173934797756,"endowment":4.762173934797756,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7858270","pmcid":null,"openalex_id":"https://openalex.org/W2264631436","authors":[],"funders":[{"funder_name":"NEI NIH HHS","grant_id":"EY06337","title":null}],"total_grants":1,"fwci":8.4733,"citation_percentile":0.980095,"influential_citations":5,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":1},{"year":2014,"count":1},{"year":2015,"count":3},{"year":2017,"count":3},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2026,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://ashpublications.org/blood/article-pdf/85/5/1396/615420/1396.pdf","host_type":"journal"},{"url":"https://ashpublications.org/blood/article-pdf/85/5/1396/615420/1396.pdf","host_type":"BRONZE"},{"url":"https://ashpublications.org/blood/article-pdf/85/5/1396/615420/1396.pdf","host_type":"publisher"},{"url":"http://ashpublications.org/blood/article-pdf/85/5/1396/615420/1396.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1182/blood.v85.5.1396.bloodjournal8551396","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7858270","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.921.8021","host_type":""}],"fields_of_study":["Viral-associated cancers and disorders","Lymphoma Diagnosis and Treatment","Polyomavirus and related diseases","Medicine","Biology"],"mesh_terms":["Antigens, Viral","B-Lymphocytes","Base Sequence","Cell Line, Transformed","DNA-Binding Proteins","DNA, Viral","Herpesvirus 4, Human","Genes, Viral","Humans","Lymphoproliferative Disorders","Molecular Sequence Data","Postoperative Complications","Transplantation","Virulence","Viral Structural Proteins","Polymerase Chain Reaction","Immunocompromised Host","Polymorphism, Single-Stranded Conformational","Epstein-Barr Virus Nuclear Antigens"],"keywords":["Lymphoproliferative disorders","Biology","Epstein–Barr virus","Virus","Virology","Gammaherpesvirinae","Polymerase chain reaction","Herpesviridae","Lymphoma","Transplantation","Immunology","Epstein–Barr virus infection","Gene","Viral disease","Genetics","Medicine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T06:55:32.887066Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}