{"doi":"10.1182/blood.2020005780","title":"Dynamic CTCF binding directly mediates interactions among <i>cis</i>-regulatory elements essential for hematopoiesis","abstract":"While constitutive CCCTC-binding factor (CTCF)-binding sites are needed to maintain relatively invariant chromatin structures, such as topologically associating domains, the precise roles of CTCF to control cell-type-specific transcriptional regulation remain poorly explored. We examined CTCF occupancy in different types of primary blood cells derived from the same donor to elucidate a new role for CTCF in gene regulation during blood cell development. We identified dynamic, cell-type-specific binding sites for CTCF that colocalize with lineage-specific transcription factors. These dynamic sites are enriched for single-nucleotide polymorphisms that are associated with blood cell traits in different linages, and they coincide with the key regulatory elements governing hematopoiesis. CRISPR-Cas9-based perturbation experiments demonstrated that these dynamic CTCF-binding sites play a critical role in red blood cell development. Furthermore, precise deletion of CTCF-binding motifs in dynamic sites abolished interactions of erythroid genes, such as RBM38, with their associated enhancers and led to abnormal erythropoiesis. These results suggest a novel, cell-type-specific function for CTCF in which it may serve to facilitate interaction of distal regulatory emblements with target promoters. Our study of the dynamic, cell-type-specific binding and function of CTCF provides new insights into transcriptional regulation during hematopoiesis.","journal":"Blood","year":2020,"id":91271,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":46,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9651,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":457177,"name":"Cheng Li","orcid":"0000-0002-1286-9403","position":1,"is_corresponding":false},{"id":457178,"name":"Xing Tang","orcid":"0000-0001-5570-2155","position":2,"is_corresponding":false},{"id":457179,"name":"Yanghua He","orcid":"0000-0001-6849-6493","position":3,"is_corresponding":false},{"id":12577,"name":"Yichao Li","orcid":"0000-0001-5791-096X","position":4,"is_corresponding":false},{"id":457180,"name":"Tiffany Yee","orcid":"0000-0002-8709-8017","position":5,"is_corresponding":false},{"id":457181,"name":"Dewan Shrestha","orcid":"0000-0003-2831-2498","position":6,"is_corresponding":false},{"id":458105,"name":"Ruopeng Feng","orcid":null,"position":7,"is_corresponding":false},{"id":457182,"name":"Peng Xu","orcid":"0000-0002-7573-4813","position":8,"is_corresponding":false},{"id":252141,"name":"Xin Zhou","orcid":"0000-0003-3979-8200","position":9,"is_corresponding":false},{"id":250571,"name":"Shondra M. Pruett‐Miller","orcid":"0000-0002-3793-585X","position":10,"is_corresponding":false},{"id":19594,"name":"Ross C. Hardison","orcid":"0000-0003-4084-7516","position":11,"is_corresponding":false},{"id":90394,"name":"Mitchell J. Weiss","orcid":"0000-0003-2460-3036","position":12,"is_corresponding":false},{"id":36425,"name":"Yong Cheng","orcid":"0000-0002-9314-0971","position":13,"is_corresponding":false},{"id":351516,"name":"Qian Qi","orcid":"0000-0002-9853-0936","position":0,"is_corresponding":true}],"reference_count":74,"raw_metadata":null,"created_at":"2026-07-18T22:28:45.409789Z","pmid":"33512425","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}