{"doi":"10.1177/29.4.6166661","title":"Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.","abstract":"The use of avidin-biotin interaction in immunoenzymatic techniques provides a simple and sensitive method to localize antigens in formalin-fixed tissues. Among the several staining procedures available, the ABC method, which involves an application of biotin-labeled secondary antibody followed by the addition of avidin-biotin-peroxidase complex, gives a superior result when compared to the unlabeled antibody method. The availability of biotin-binding sites in the complex is created by the incubation of a relative excess of avidin with biotin-labeled peroxidase. During formation of the complex, avidin acts as a bridge between biotin-labeled peroxidase molecules; and biotin-labeled peroxidase molecules, which contains several biotin moieties, serve as a link between the avidin molecules. Consequently, a \"lattice\" complex containing several peroxidase molecules is likely formed. Binding of this complex to the biotin moieties associated with secondary antibody results in a high staining intensity.","journal":"Journal of Histochemistry & Cytochemistry","year":1981,"id":9057,"datarank":30.949121931678103,"base_score":9.564512185696797,"endowment":9.564512185696797,"self_citation_contribution":1.4346768278545199,"citation_network_contribution":29.514445103823583,"self_endowment_contribution":1.4346768278545199,"citer_contribution":29.514445103823583,"corpus_percentile":99.2,"corpus_rank":696,"citation_count":14249,"citer_count":198,"citers_with_citation_signal":198,"citers_with_endowment":198,"datacite_reuse_total":0,"is_dataset":false,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"1981-04-01","authors":[{"id":77874,"name":"L Raine","orcid":null,"position":1,"is_corresponding":false},{"id":77875,"name":"H Fanger","orcid":null,"position":2,"is_corresponding":false},{"id":77876,"name":"Laurence Raine","orcid":null,"position":3,"is_corresponding":false},{"id":77877,"name":"Herbert Fanger","orcid":null,"position":4,"is_corresponding":false},{"id":77873,"name":"S M Hsu","orcid":null,"position":0,"is_corresponding":true}],"reference_count":10,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}