{"doi":"10.1177/25152564221125613","title":"VPS13A and VPS13C Influence Lipid Droplet Abundance","abstract":"<jats:p>Lipid transfer proteins mediate the exchange of lipids between closely apposed membranes at organelle contact sites and play key roles in lipid metabolism, membrane homeostasis, and cellular signaling. A recently discovered novel family of lipid transfer proteins, which includes the VPS13 proteins (VPS13A-D), adopt a rod-like bridge conformation with an extended hydrophobic groove that enables the bulk transfer of membrane lipids for membrane growth. Loss of function mutations in VPS13A and VPS13C cause chorea acanthocytosis and Parkinson's disease, respectively. VPS13A and VPS13C localize to multiple organelle contact sites, including endoplasmic reticulum (ER) – lipid droplet (LD) contact sites, but the functional roles of these proteins in LD regulation remains mostly unexplored. Here we employ CRISPR-Cas9 genome editing to generate VPS13A and VPS13C knockout cell lines in U-2 OS cells via deletion of exon 2 and introduction of an early frameshift. Analysis of LD content in these cell lines revealed that loss of either VPS13A or VPS13C results in reduced LD abundance under oleate-stimulated conditions. These data implicate two lipid transfer proteins, VPS13A and VPS13C, in LD regulation.</jats:p>","journal":"Contact","year":2022,"id":30739,"datarank":0.9058016222702685,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.4354774898808959,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.4354774898808959,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":21,"citers_with_citation_signal":18,"citers_with_endowment":18,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":166309,"name":"Melissa A. Roberts","orcid":null,"position":1,"is_corresponding":false},{"id":166310,"name":"Chun-Yuan Chen","orcid":"0000-0001-6212-2888","position":2,"is_corresponding":false},{"id":166311,"name":"Sebastian Markmiller","orcid":null,"position":3,"is_corresponding":false},{"id":166312,"name":"Hong-Guang Wei","orcid":"0000-0003-1332-9350","position":4,"is_corresponding":false},{"id":43218,"name":"Gene W. Yeo","orcid":"0000-0002-0799-6037","position":5,"is_corresponding":false},{"id":166317,"name":"James G. Granneman","orcid":null,"position":6,"is_corresponding":false},{"id":166314,"name":"James A. Olzmann","orcid":"0000-0001-7751-8316","position":7,"is_corresponding":false},{"id":121778,"name":"Susan Ferro-Novick","orcid":"0000-0001-8714-7352","position":8,"is_corresponding":false},{"id":121773,"name":"Shuliang Chen","orcid":"0000-0002-7175-7604","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36147729","pmcid":"PMC9491623","openalex_id":"https://openalex.org/W4296799093","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"R01DK076629","title":null},{"funder_name":"National Institutes of Health","grant_id":"R01DK128099","title":null},{"funder_name":"National Institutes of Health","grant_id":"R01GM112948","title":null},{"funder_name":"National Institutes of 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Institutes of Health","grant_id":"5R01DK128099-03","title":"Lipid droplet regulation and proteome dynamics"},{"funder_name":"National Institutes of Health","grant_id":"5U24HG009889-07","title":"A comprehensive binding and functional map of human RNA-binding proteins"},{"funder_name":"National Institutes of Health","grant_id":"3R35GM131681-03S1","title":"The diverse roles of ER-Golgi trafficking machinery in autophagy and ER quality control"},{"funder_name":"National Institutes of Health","grant_id":"2R01DK076629-07A1","title":"Analysis of Lipolytic Trafficking in Adipocytes"},{"funder_name":"National Institutes of Health","grant_id":"1R01NS117440-01","title":"The connection between ER-phagy, ER structure and hereditary spastic paraplegias"}],"total_grants":19,"fwci":2.5027,"citation_percentile":0.88237037,"influential_citations":0,"citation_trend":[{"year":2023,"count":6},{"year":2024,"count":7},{"year":2025,"count":6},{"year":2026,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.sagepub.com/doi/reader/10.1177/25152564221125613","host_type":"journal"},{"url":"https://journals.sagepub.com/doi/reader/10.1177/25152564221125613","host_type":"GOLD"},{"url":"https://journals.sagepub.com/doi/reader/10.1177/25152564221125613","host_type":"publisher"},{"url":"http://journals.sagepub.com/doi/pdf/10.1177/25152564221125613","host_type":"publisher"},{"url":"http://journals.sagepub.com/doi/full-xml/10.1177/25152564221125613","host_type":"publisher"},{"url":"https://doi.org/10.1177/25152564221125613","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36147729","host_type":"repository"},{"url":"https://doaj.org/article/1e1a9db2ba5f434ba1ddfd7413e4ae42","host_type":"repository"},{"url":"https://escholarship.org/uc/item/12v7z12b","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9491623","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9491623","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9491623?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1101/2022.06.21.497109","host_type":""},{"url":"http://dx.doi.org/10.1177/25152564221125613","host_type":""},{"url":"https://escholarship.org/content/qt12v7z12b/qt12v7z12b.pdf","host_type":""},{"url":"https://doi.org/https://doi.org/10.1177/25152564221125613","host_type":""}],"fields_of_study":["Lipid metabolism and biosynthesis","Endoplasmic Reticulum Stress and Disease","Pancreatic function and diabetes","Biology","Medicine","0301 basic medicine","03 medical and health sciences"],"mesh_terms":[],"keywords":["Organelle","Membrane contact site","Plant lipid transfer proteins","Endoplasmic reticulum","Cell biology","Lipid droplet","Biology","Lipid metabolism","Microsomal triglyceride transfer protein","Membrane protein","Membrane lipids","Chemistry","Biochemistry","Membrane","Integral membrane protein","Gene","Cholesterol","Lipoprotein","Lipid transfer protein","contact","1.1 Normal biological development and functioning (hrcs-rac)","570","31 Biological Sciences (for-2020)","QH301-705.5","1.1 Normal biological development and functioning","Generic health relevance (hrcs-hc)","610","2.1 Biological and endogenous factors (hrcs-rac)","QD415-436","Biological Sciences","VPS13 and Bridge-like Lipid Transfer Proteins: A New Mode of Intracellular Continuity – Report","3101 Biochemistry and 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