{"doi":"10.1177/10998004251411301","title":"Estrogen Stabilizes Interferon-Inducible Genes During Skeletal Muscle Regeneration","abstract":"Background: Inflammation is essential for skeletal muscle repair following acute injury, and successful muscle regeneration requires a balance between pro- and anti-inflammatory signaling. Estrogen regulates inflammatory responses, suggesting a role in tissue repair. CD169+ macrophages are also associated with tissue repair. However, these cells and related interferon-inducible genes have not been investigated in regenerating muscle. Objective: To examine how 17β-estradiol treatment influences the expression and co-expression network of Siglec1 (encoding CD169), an interferon-inducible gene, during muscle regeneration. Methods: Ovariectomized mice received either 17β-estradiol or placebo before undergoing a standardized crush injury to hindlimb muscle groups and exposure to simulated flight. Mice were euthanized at 32-h, 96-h, or 192-h postinjury. Differential gene expression analysis was performed on injured muscles to assess treatment-related transcriptional changes, and additional muscles were evaluated for regeneration stage and CD169 protein expression. Results: At 192-h postinjury, Siglec1 was among the top upregulated genes in 17β-estradiol-treated mice relative to placebo mice, which reflected a decline in Siglec1 expression over time in the placebo group. Correlation analysis revealed that Siglec1 was strongly associated with interferon-related genes under placebo treatment, whereas the connectivity of Siglec1 under 17β-estradiol treatment was weakened. Immunohistochemistry confirmed stronger CD169 staining in regenerating muscle of 17β-estradiol-treated mice. Conclusion: Siglec1 expression is more stable in an estrogen state compared to an ovarian-hormone-deficient state. Understanding how estrogen deficiency alters inflammatory signaling after muscle injury may inform interventions to promote recovery in postmenopausal women, who may be at risk for impaired muscle repair.","journal":"Biological Research For Nursing","year":2025,"id":587220,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9546,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":512245,"name":"Juli Petereit","orcid":"0000-0002-8017-3280","position":1,"is_corresponding":false},{"id":1502883,"name":"Barbara Anne Schneider","orcid":null,"position":2,"is_corresponding":false},{"id":1502882,"name":"Tara Movaghar","orcid":null,"position":0,"is_corresponding":true}],"reference_count":29,"raw_metadata":null,"created_at":"2026-07-19T02:59:36.020030Z","pmid":"41454847","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}