{"doi":"10.1177/108705719900400206","title":"A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays","abstract":"The ability to identify active compounds (³hits²) from large chemical libraries accurately and rapidly has been the ultimate goal in developing high-throughput screening (HTS) assays. The ability to identify hits from a particular HTS assay depends largely on the suitability or quality of the assay used in the screening. The criteria or parameters for evaluating the ³suitability² of an HTS assay for hit identification are not well defined and hence it still remains difficult to compare the quality of assays directly. In this report, a screening window coefficient, called ³Z-factor,² is defined. This coefficient is reflective of both the assay signal dynamic range and the data variation associated with the signal measurements, and therefore is suitable for assay quality assessment. The Z-factor is a dimensionless, simple statistical characteristic for each HTS assay. The Z-factor provides a useful tool for comparison and evaluation of the quality of assays, and can be utilized in assay optimization and validation.","journal":"SLAS Discovery","year":1999,"id":11736,"datarank":21.92483553501376,"base_score":8.84246002419529,"endowment":8.84246002419529,"self_citation_contribution":1.3263690036292937,"citation_network_contribution":20.598466531384467,"self_endowment_contribution":1.3263690036292937,"citer_contribution":20.598466531384467,"corpus_percentile":94.4,"corpus_rank":1720,"citation_count":6921,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"1999-04-01","authors":[{"id":94444,"name":"Thomas D.Y. Chung","orcid":null,"position":1,"is_corresponding":false},{"id":94445,"name":"Kevin R. Oldenburg","orcid":"0000-0002-5916-673X","position":2,"is_corresponding":false},{"id":94443,"name":"Ji-Hu Zhang","orcid":null,"position":0,"is_corresponding":true}],"reference_count":8,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}