{"doi":"10.1177/1040638714523609","title":"Evaluation of a disintegrin-like and metalloprotease with thrombospondin type 1 repeat motifs 13 (ADAMTS13) activity enzyme-linked immunosorbent assay for measuring plasma ADAMTS13 activity in dogs","abstract":"<jats:p>A disintegrin-like and metalloprotease with thrombospondin type 1 repeat motifs 13 (ADAMTS13) is a von Willebrand factor (vWF)-cleaving protease. Deficiencies in ADAMTS13 activity are known to cause thrombotic diseases in human beings. The present study evaluated whether the human ADAMTS13 activity enzyme-linked immunosorbent assay (ELISA) kit containing human vWF73 (a minimal substrate) and anti-N10 antibody (which specifically recognizes the decapeptide of the C-terminal edge of cleaved vWF by human ADAMTS13) is applicable to the measurement of canine plasma ADAMTS13 activity. Human vWF73 fused with a GST-tag and a His-tag (GST-hvWF73-His) was reacted with recombinant canine (rc)ADAMTS13, canine plasma, and human plasma, and then used in Western blotting using anti-N10 antibody. Linearity and intra- and interassay reproducibility of the human ADAMTS13 activity ELISA kit in canine plasma were further evaluated. Finally, plasma ADAMTS13 activity was measured in 13 healthy dogs and 6 dogs with bacteremia using the human ADAMTS13 activity ELISA kit. Cleaved products with a 28-kDa GST-hvWF73-His were detected specifically in rcADAMTS13 as well as in human ADAMTS13, and also in canine plasma by anti-N10 antibody, showing excellent linearity. Intra-assay coefficient of variation (CV) was 3.0–12.4%, and interassay CV was 11.5–12.5%. The ADAMTS13 activity was significantly lower in dogs with bacteremia than in healthy dogs ( P = 0.0025). The current study revealed that the human ADAMTS13 activity ELISA kit is applicable for measurement of canine plasma ADAMTS13 activity to elucidate the pathology of thrombotic diseases in dogs.</jats:p>","journal":"Journal of Veterinary Diagnostic Investigation","year":2014,"id":19889,"datarank":0.23483491356962588,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.026890759401642265,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.026890759401642265,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":3,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":133323,"name":"Michiko Kaneko","orcid":null,"position":1,"is_corresponding":false},{"id":133324,"name":"Taiga Otake","orcid":null,"position":2,"is_corresponding":false},{"id":133325,"name":"Rui Kano","orcid":null,"position":3,"is_corresponding":false},{"id":133326,"name":"Yoshiki Yamaya","orcid":null,"position":4,"is_corresponding":false},{"id":133327,"name":"Toshihiro Watari","orcid":null,"position":5,"is_corresponding":false},{"id":133328,"name":"Atsuhiko Hasegawa","orcid":null,"position":6,"is_corresponding":false},{"id":133329,"name":"Hiroshi Kamata","orcid":null,"position":7,"is_corresponding":false},{"id":133322,"name":"Haruhiko Maruyama","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24583948","pmcid":null,"openalex_id":"https://openalex.org/W2162228301","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.15375753,"influential_citations":0,"citation_trend":[{"year":2020,"count":1},{"year":2022,"count":1},{"year":2025,"count":1}],"oa_status":"bronze","license":"cc-by-nc-nd","oa_locations":[{"url":"https://journals.sagepub.com/doi/pdf/10.1177/1040638714523609","host_type":"journal"},{"url":"https://journals.sagepub.com/doi/pdf/10.1177/1040638714523609","host_type":"BRONZE"},{"url":"https://journals.sagepub.com/doi/pdf/10.1177/1040638714523609","host_type":"publisher"},{"url":"https://journals.sagepub.com/doi/full-xml/10.1177/1040638714523609","host_type":"publisher"},{"url":"https://doi.org/10.1177/1040638714523609","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24583948","host_type":"repository"},{"url":"https://teikyo-u.repo.nii.ac.jp/records/2014826","host_type":"repository"}],"fields_of_study":["Complement system in diseases","Platelet Disorders and Treatments","Blood Coagulation and Thrombosis Mechanisms","Chemistry","Medicine","ADAM Proteins","Animals","Bacteremia","Blotting, Western","Dogs","Enzyme-Linked Immunosorbent Assay","Gene Expression Regulation","HeLa Cells","Humans","Protein Subunits","Reproducibility of Results","von Willebrand Factor"],"mesh_terms":["Animals","Dogs","Enzyme-Linked Immunosorbent Assay","Gene Expression Regulation","HeLa Cells","Humans","von Willebrand Factor","Blotting, Western","Reproducibility of Results","Bacteremia","Protein Subunits","ADAM Proteins","Hela Cells"],"keywords":["Thrombospondin","Disintegrin","Metalloproteinase","ADAMTS13","Enzyme","Thrombospondin 1","Molecular biology","ADAMTS","Biology","Chemistry","Immunology","Biochemistry","Genetics","Angiogenesis","Dogs","Anti-n10 Antibody","Disintegrin-like And Metalloprotease With Thrombospondin Type 1 Repeat Motifs 13","Von Willebrand Factor 73"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-04T06:09:54.845515Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}