{"doi":"10.1172/jci170173","title":"The CTBP2-PCIF1 complex regulates m6Am modification of mRNA in head and neck squamous cell carcinoma","abstract":null,"journal":"Journal of Clinical Investigation","year":2023,"id":647010,"datarank":0.515098080672772,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"self_citation_contribution":0.515098080672772,"citation_network_contribution":0.0,"self_endowment_contribution":0.515098080672772,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1435278,"name":"Jie Chen","orcid":"0000-0001-7925-3729","position":1,"is_corresponding":false},{"id":1652630,"name":"Caihua Zhang","orcid":null,"position":2,"is_corresponding":false},{"id":1652627,"name":"Maosheng Cheng","orcid":null,"position":3,"is_corresponding":false},{"id":1484041,"name":"Shuang Chen","orcid":"0009-0005-4968-5869","position":4,"is_corresponding":false},{"id":636520,"name":"Wei Song","orcid":"0000-0002-7007-5281","position":5,"is_corresponding":false},{"id":1685499,"name":"Chunlong Yang","orcid":null,"position":6,"is_corresponding":false},{"id":1685500,"name":"Rongsong Ling","orcid":"0000-0002-0402-9818","position":7,"is_corresponding":false},{"id":1338023,"name":"Zhi Chen","orcid":"0000-0002-4385-1484","position":8,"is_corresponding":false},{"id":311121,"name":"Xiaochen Wang","orcid":"0000-0001-8911-3690","position":9,"is_corresponding":false},{"id":1652631,"name":"Gan Xiong","orcid":null,"position":10,"is_corresponding":false},{"id":1685501,"name":"Jieyi Ma","orcid":null,"position":11,"is_corresponding":false},{"id":319654,"name":"Yan Zhu","orcid":"0000-0001-8692-0817","position":12,"is_corresponding":false},{"id":1349020,"name":"Quan Yuan","orcid":"0000-0002-3085-431X","position":13,"is_corresponding":false},{"id":430244,"name":"Qi Liu","orcid":"0000-0002-5684-7396","position":14,"is_corresponding":false},{"id":720661,"name":"Liang Peng","orcid":"0000-0002-2088-1904","position":15,"is_corresponding":false},{"id":668,"name":"Qianming Chen","orcid":"0000-0002-5371-4432","position":16,"is_corresponding":false},{"id":630258,"name":"Demeng Chen","orcid":"0000-0001-7230-2495","position":17,"is_corresponding":false},{"id":431014,"name":"Kang Li","orcid":"0000-0003-1893-4074","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The CTBP2-PCIF1 complex regulates m6Am modification of mRNA in head and neck squamous cell carcinoma","abstract":"PCIF1 can mediate the methylation of N6,2'-O-dimethyladenosine (m6Am) in mRNA. Yet, the detailed interplay between PCIF1 and the potential cofactors and its pathological significance remain elusive. Here, we demonstrated that PCIF1-mediated cap mRNA m6Am modification promoted head and neck squamous cell carcinoma progression both in vitro and in vivo. CTBP2 was identified as a cofactor of PCIF1 to catalyze m6Am deposition on mRNA. CLIP-Seq data demonstrated that CTBP2 bound to similar mRNAs as compared with PCIF1. We then used the m6Am-Seq method to profile the mRNA m6Am site at single-base resolution and found that mRNA of TET2, a well-known tumor suppressor, was a major target substrate of the PCIF1-CTBP2 complex. Mechanistically, knockout of CTBP2 reduced PCIF1 occupancy on TET2 mRNA, and the PCIF1-CTBP2 complex negatively regulated the translation of TET2 mRNA. Collectively, our study demonstrates the oncogenic function of the epitranscriptome regulator PCIF1-CTBP2 complex, highlighting the importance of the m6Am modification in tumor progression.","is_dataset_classified":null,"base_score":3.4011973816621555,"endowment":3.4011973816621555,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37643007","pmcid":"PMC10575729","openalex_id":"https://openalex.org/W4386250314","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"82173362,81872409","title":null},{"funder_name":"Guangdong Basic and Applied Basic Research Foundation","grant_id":"2019A1515110110","title":null},{"funder_name":"China Postdoctoral Science Foundation","grant_id":"2022M720175","title":null}],"total_grants":3,"fwci":2.6983,"citation_percentile":0.91450394,"influential_citations":0,"citation_trend":[{"year":2024,"count":10},{"year":2025,"count":12},{"year":2026,"count":7}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"http://www.jci.org/articles/view/170173/files/pdf","host_type":"journal"},{"url":"https://www.jci.org/articles/view/170173/files/pdf","host_type":"publisher"},{"url":"https://doi.org/10.1172/jci170173","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/37643007","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10575729","host_type":"repository"},{"url":"https://doaj.org/article/a6cdb4b0a9054f85a64d0bded0acf3a9","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC10575729","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC10575729?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["RNA modifications and cancer","Cancer-related gene regulation","RNA Research and Splicing","Humans","Adaptor Proteins, Signal Transducing","Alcohol Oxidoreductases","Co-Repressor Proteins","Head and Neck Neoplasms","Methylation","Nuclear Proteins","RNA, Messenger","Squamous Cell Carcinoma of Head and Neck","Transcription Factors","Adenosine"],"mesh_terms":["Squamous Cell Carcinoma of Head and Neck","Adenosine","Alcohol Oxidoreductases","Head and Neck Neoplasms","Humans","Methylation","Nuclear Proteins","RNA, Messenger","Transcription Factors","Adaptor Proteins, Signal Transducing","Co-Repressor Proteins"],"keywords":["Messenger RNA","Biology","Translation (biology)","Methylation","Head and neck squamous-cell carcinoma","Cell biology","Molecular biology","Genetics","Gene","Cancer","Head and neck cancer","Oncology","Epigenetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"refseq"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-09T16:13:48.473694Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}