{"doi":"10.1172/jci153920","title":"Sepsis promotes splenic production of a protective platelet pool with high CD40 ligand expression","abstract":"Platelets have a wide range of functions including critical roles in hemostasis, thrombosis, and immunity. We hypothesized that during acute inflammation, such as in life-threatening sepsis, there are fundamental changes in the sites of platelet production and phenotypes of resultant platelets. Here, we showed during sepsis that the spleen was a major site of megakaryopoiesis and platelet production. Sepsis provoked an adrenergic-dependent mobilization of megakaryocyte-erythrocyte progenitors (MEPs) from the bone marrow to the spleen, where IL-3 induced their differentiation into megakaryocytes (MKs). In the spleen, immune-skewed MKs produced a CD40 ligandhi platelet population with potent immunomodulatory functions. Transfusions of post-sepsis platelets enriched from splenic production enhanced immune responses and reduced overall mortality in sepsis-challenged animals. These findings identify a spleen-derived protective platelet population that may be broadly immunomodulatory in acute inflammatory states such as sepsis.","journal":"Journal of Clinical Investigation","year":2022,"id":234380,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":99,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9467,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":322388,"name":"Mélia Magnen","orcid":"0000-0001-5189-0072","position":1,"is_corresponding":false},{"id":342876,"name":"Longhui Qiu","orcid":"0000-0003-3122-9679","position":2,"is_corresponding":false},{"id":251403,"name":"Simon J. Cleary","orcid":"0000-0001-5573-6363","position":3,"is_corresponding":false},{"id":348614,"name":"Kristin M. Wang","orcid":"0000-0002-2108-9900","position":4,"is_corresponding":false},{"id":348613,"name":"Serena Ranucci","orcid":"0000-0001-7177-2188","position":5,"is_corresponding":false},{"id":850445,"name":"Élodie Grockowiak","orcid":null,"position":6,"is_corresponding":false},{"id":754478,"name":"Rafik Boudra","orcid":"0000-0003-3211-0127","position":7,"is_corresponding":false},{"id":678612,"name":"Catharina Conrad","orcid":"0000-0002-7160-9169","position":8,"is_corresponding":false},{"id":849909,"name":"Yurim Seo","orcid":"0009-0002-1281-0919","position":9,"is_corresponding":false},{"id":311012,"name":"Daniel R. Calabrese","orcid":"0000-0002-0596-3434","position":10,"is_corresponding":false},{"id":311020,"name":"John R. Greenland","orcid":"0000-0003-1422-8367","position":11,"is_corresponding":false},{"id":597430,"name":"Andrew D. Leavitt","orcid":"0000-0003-4827-0790","position":12,"is_corresponding":false},{"id":29970,"name":"Emmanuelle Passegué","orcid":"0000-0002-3516-297X","position":13,"is_corresponding":false},{"id":589437,"name":"Simón Méndez‐Ferrer","orcid":"0000-0002-9805-9988","position":14,"is_corresponding":false},{"id":248978,"name":"Filip K. Świrski","orcid":"0000-0002-3163-9152","position":15,"is_corresponding":false},{"id":251402,"name":"Mark R. Looney","orcid":"0000-0003-0241-9190","position":16,"is_corresponding":false},{"id":349669,"name":"Colin Valet","orcid":null,"position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-19T00:21:36.529874Z","pmid":"35192546","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}