{"doi":"10.1172/jci117162","title":"Activation of H+ conductance in neutrophils requires assembly of components of the respiratory burst oxidase but not its redox function.","abstract":null,"journal":"Journal of Clinical Investigation","year":1994,"id":673552,"datarank":0.5375278407684165,"base_score":3.58351893845611,"endowment":3.58351893845611,"self_citation_contribution":0.5375278407684165,"citation_network_contribution":0.0,"self_endowment_contribution":0.5375278407684165,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":35,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1759768,"name":"J T Curnutte","orcid":null,"position":1,"is_corresponding":false},{"id":1759772,"name":"S Grinstein","orcid":null,"position":2,"is_corresponding":false},{"id":1759766,"name":"A Nanda","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Activation of H+ conductance in neutrophils requires assembly of components of the respiratory burst oxidase but not its redox function.","abstract":"In phagocytes, superoxide generation by the NADPH oxidase is accompanied by metabolic acid production. Cytoplasmic acidification during this metabolic burst is prevented by a combination of H+ extrusion mechanisms, including a unique H+ conductance. NADPH oxidase is deficient in chronic granulomatous disease (CGD) patients. The burst of acid production is absent in CGD patients lacking the 47-kD (p47-phox) or the 91-kD (gp91-phox) subunits of the oxidase. Activation of the H+ conductance is also defective in these patients suggesting that (a) the oxidase itself undertakes H+ translocation or (b) oxidase assembly is required to stimulate a separate H+ conducting entity. To discern between these possibilities, three rare forms of CGD were studied. In neutrophils expressing nonfunctional cytochrome b, the conductance was activated to near-normal levels, implying that functional oxidase is not required to activate H+ extrusion. CGD cells expressing diminished amounts of cytochrome displayed H+ conductance approaching normal levels, suggesting that the oxidase itself does not translocate H+. Finally, the conductance was only partially inhibited in patients lacking the 67-kD subunit, indicating that this component is not essential for stimulation of H+ transport. We propose that normal assembly of the oxidase subunits is required for optimal activation of a closely associated but distinct H+ conducting entity.","is_dataset_classified":null,"base_score":3.58351893845611,"endowment":3.58351893845611,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8163676","pmcid":"PMC294241","openalex_id":"https://openalex.org/W2038723032","authors":[],"funders":[{"funder_name":"NCRR NIH HHS","grant_id":"RR00833","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI24838","title":null}],"total_grants":2,"fwci":0.7955,"citation_percentile":0.69691822,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2014,"count":1},{"year":2015,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"http://www.jci.org/articles/view/117162/files/pdf","host_type":"journal"},{"url":"http://www.jci.org/articles/view/117162/files/pdf","host_type":"publisher"},{"url":"https://doi.org/10.1172/jci117162","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8163676","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/294241","host_type":"repository"}],"fields_of_study":["Ion channel regulation and function","Neuroscience of respiration and sleep","Neuroscience and Neuropharmacology Research","Female","Granulomatous Disease, Chronic","Humans","Ion Transport","Male","NADH, NADPH Oxidoreductases","NADPH Oxidases","Neutrophils","Oxidation-Reduction","Point Mutation","Protons","Respiratory Burst","Tetradecanoylphorbol Acetate"],"mesh_terms":["Female","Granulomatous Disease, Chronic","Humans","Male","NADH, NADPH Oxidoreductases","Neutrophils","Oxidation-Reduction","Protons","Tetradecanoylphorbol Acetate","Respiratory Burst","Ion Transport","Point Mutation","NADPH Oxidases"],"keywords":["NADPH oxidase","Oxidase test","Chronic granulomatous disease","Respiratory burst","Superoxide","Cytochrome c oxidase","Chemistry","Biochemistry","Protein subunit","Alternative oxidase","Enzyme","Biophysics","Biology","Immunology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T13:46:23.556563Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}