{"doi":"10.1172/jci110850","title":"Comparison of glucosylated low density lipoprotein with methylated or cyclohexanedione-treated low density lipoprotein in the measurement of receptor-independent low density lipoprotein catabolism.","abstract":null,"journal":"Journal of Clinical Investigation","year":1983,"id":633461,"datarank":0.6515708132780527,"base_score":4.343805421853684,"endowment":4.343805421853684,"self_citation_contribution":0.6515708132780527,"citation_network_contribution":0.0,"self_endowment_contribution":0.6515708132780527,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":76,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1642435,"name":"J L Witztum","orcid":null,"position":1,"is_corresponding":false},{"id":1642436,"name":"Y A Kesaniemi","orcid":null,"position":2,"is_corresponding":false},{"id":1642437,"name":"R L Elam","orcid":null,"position":3,"is_corresponding":false},{"id":1642434,"name":"U P Steinbrecher","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Comparison of glucosylated low density lipoprotein with methylated or cyclohexanedione-treated low density lipoprotein in the measurement of receptor-independent low density lipoprotein catabolism.","abstract":"We previously showed that glucosylation of lysine residues of low density lipoproteins (LDL) blocks high-affinity degradation by cultured human fibroblasts, and markedly slows LDL turnover in guinea pigs. The present studies were done to evaluate glucosylated (GLC) LDL as a tracer of receptor-independent LDL catabolism, and to compare it with two other modified LDL, methylated (MET) LDL, and cyclohexanedione (CHD)-treated LDL, which have been used previously for this purpose. Glucosylation of LDL did not affect receptor-independent degradation in vivo, as the turnover of GLC-LDL and native LDL were similar in the LDL receptor-deficient, Watanabe heritable hyperlipidemic rabbit. Each modified radiolabeled LDL preparation was injected into eight guinea pigs, and fractional catabolic rates (FCR) determined. The FCR of GLC-LDL (0.024 +/- 0.005 h-1; SD) was similar to that of MET-LDL (0.023 +/- 0.006 h-1), and approximately 22% of that of native LDL (0.105 +/- 0.02 h-1). The FCR of CHD-LDL was greater than that of the other modified LDL, and it varied depending on how soon after preparation the CHD-LDL was injected: when used within 2 h of preparation, the mean FCR was 0.044 +/- 0.007 h-1 (n = 4); when used after overnight dialysis at 4 degrees C, the mean FCR was 0.082 +/- 0.03 h-1 (n = 4). This suggests that CHD-LDL overestimates the amount of LDL degraded by receptor-independent pathways, perhaps because the CHD modification is spontaneously reversible. The present studies indicate that GLC-LDL is a useful tracer of receptor-independent LDL catabolism in animals.","is_dataset_classified":null,"base_score":4.343805421853684,"endowment":4.343805421853684,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"6300195","pmcid":"PMC436953","openalex_id":"https://openalex.org/W2044679041","authors":[],"funders":[{"funder_name":"NHLBI NIH HHS","grant_id":"HL 14197","title":null}],"total_grants":1,"fwci":13.9318,"citation_percentile":0.99173982,"influential_citations":0,"citation_trend":[{"year":2017,"count":1},{"year":2026,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"http://www.jci.org/articles/view/110850/files/pdf","host_type":"journal"},{"url":"http://www.jci.org/articles/view/110850/files/pdf","host_type":"publisher"},{"url":"https://doi.org/10.1172/jci110850","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/6300195","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/436953","host_type":"repository"}],"fields_of_study":["Lipoproteins and Cardiovascular Health","Cholesterol and Lipid Metabolism","Diabetes, Cardiovascular Risks, and Lipoproteins","Animals","Cyclohexanones","Fibroblasts","Glucose","Guinea Pigs","Humans","Infant","Kinetics","Lipoproteins, LDL","Male","Methylation","Rabbits","Receptors, Cell Surface","Receptors, LDL"],"mesh_terms":["Animals","Cyclohexanones","Fibroblasts","Glucose","Guinea Pigs","Humans","Infant","Kinetics","Lipoproteins, LDL","Male","Methylation","Rabbits","Receptors, Cell Surface","Receptors, LDL"],"keywords":["Catabolism","LDL receptor","Low-density lipoprotein","Chemistry","Internal medicine","Lipoprotein","Receptor","Endocrinology","Lysine","Biochemistry","Metabolism","Cholesterol","Amino acid","Biology","Medicine"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T12:07:05.434755Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}