{"doi":"10.1172/jci.insight.176208","title":"HIV-1–infected T cell clones are shared across cerebrospinal fluid and blood during ART","abstract":"The central nervous system HIV reservoir is incompletely understood and is a major barrier to HIV cure. We profiled people with HIV (PWH) and uninfected controls through single-cell transcriptomic and T cell receptor (TCR) sequencing to understand the dynamics of HIV persistence in the CNS. In PWH on ART, we found that most participants had single cells containing HIV-1 RNA, which was found predominantly in CD4 central memory T cells, in both cerebrospinal fluid (CSF) and blood. HIV-1 RNA-containing cells were found more frequently in CSF than blood, indicating a higher burden of reservoir cells in the CNS than blood for some PWH. Most CD4 T cell clones containing infected cells were compartment specific, while some (22%) - including rare clones with members of the clone containing detectable HIV RNA in both blood and CSF - were found in both CSF and blood. These results suggest that infected T cells trafficked between tissue compartments and that maintenance and expansion of infected T cell clones contributed to the CNS reservoir in PWH on ART.","journal":"JCI Insight","year":2024,"id":425448,"datarank":0.5909029774932238,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":0.14154313646012515,"self_endowment_contribution":0.4493598410330987,"citer_contribution":0.14154313646012515,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":17,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9471,"is_data_producer":true,"deposit_databanks":{"GENBANK":["K03455.1"],"GEO":["GSE243905 for C4–C6 and P1–P8"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":573992,"name":"Jennifer Yoon","orcid":"0000-0001-9225-9308","position":1,"is_corresponding":false},{"id":1116066,"name":"Hailey Reisert","orcid":"0000-0002-3606-4773","position":2,"is_corresponding":false},{"id":1223547,"name":"Bibhuprasad Das","orcid":null,"position":3,"is_corresponding":false},{"id":1223548,"name":"Benjamin Orlinick","orcid":null,"position":4,"is_corresponding":false},{"id":357316,"name":"Jennifer Chiarella","orcid":null,"position":5,"is_corresponding":false},{"id":258954,"name":"Elias K. Halvas","orcid":"0000-0002-4890-1209","position":6,"is_corresponding":false},{"id":258969,"name":"John W. Mellors","orcid":"0000-0002-3737-9742","position":7,"is_corresponding":false},{"id":69482,"name":"Alina P.S. Pang","orcid":"0000-0001-6677-7355","position":8,"is_corresponding":false},{"id":371388,"name":"Lydia Barakat","orcid":"0000-0003-4994-2907","position":9,"is_corresponding":false},{"id":1223549,"name":"Margaret Fikrig","orcid":null,"position":10,"is_corresponding":false},{"id":258961,"name":"Joshua C. Cyktor","orcid":"0000-0003-3601-4837","position":11,"is_corresponding":false},{"id":69468,"name":"Yuval Kluger","orcid":"0000-0002-3035-071X","position":12,"is_corresponding":false},{"id":69477,"name":"Serena Spudich","orcid":"0000-0001-6032-3950","position":13,"is_corresponding":false},{"id":69463,"name":"Michael J. Corley","orcid":"0000-0001-8957-7153","position":14,"is_corresponding":false},{"id":105836,"name":"Shelli Farhadian","orcid":"0000-0001-7230-1409","position":15,"is_corresponding":false},{"id":481314,"name":"Meng Wang","orcid":"0000-0002-8168-8999","position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":null,"created_at":"2026-07-19T01:58:26.927596Z","pmid":"38587074","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}