{"doi":"10.1172/jci.insight.170518","title":"Vitamin D status associates with skeletal muscle loss after anterior cruciate ligament reconstruction","abstract":"BACKGROUNDAlthough 25-hydroxyvitamin D [25(OH)D] concentrations of 30 ng/mL or higher are known to reduce injury risk and boost strength, the influence on anterior cruciate ligament reconstruction (ACLR) outcomes remains unexamined. This study aimed to define the vitamin D signaling response to ACLR, assess the relationship between vitamin D status and muscle fiber cross-sectional area (CSA) and bone density outcomes, and discover vitamin D receptor (VDR) targets after ACLR.METHODSTwenty-one young, healthy, physically active participants with recent ACL tears were enrolled (17.8 ± 3.2 years, BMI 26.0 ± 3.5 kg/m2). Data were collected through blood samples, vastus lateralis biopsies, dual energy x-ray bone density measurements, and isokinetic dynamometer measures at baseline, 1 week, 4 months, and 6 months after ACLR. The biopsies facilitated CSA, Western blotting, RNA-seq, and VDR ChIP-seq analyses.RESULTSACLR surgery led to decreased circulating bioactive vitamin D and increased VDR and activating enzyme expression in skeletal muscle 1 week after ACLR. Participants with less than 30 ng/mL 25(OH)D levels (n = 13) displayed more significant quadriceps fiber CSA loss 1 week and 4 months after ACLR than those with 30 ng/mL or higher (n = 8; P < 0.01 for post hoc comparisons; P = 0.041 for time × vitamin D status interaction). RNA-seq and ChIP-seq data integration revealed genes associated with energy metabolism and skeletal muscle recovery, potentially mediating the impact of vitamin D status on ACLR recovery. No difference in bone mineral density losses between groups was observed.CONCLUSIONCorrecting vitamin D status prior to ACLR may aid in preserving skeletal muscle during recovery.FUNDINGNIH grants R01AR072061, R01AR071398-04S1, and K99AR081367.","journal":"JCI Insight","year":2023,"id":346908,"datarank":0.38474240361923057,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.0,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9696,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":628040,"name":"Christine M. Latham","orcid":"0000-0002-0956-9688","position":1,"is_corresponding":false},{"id":1011285,"name":"Angelique N. Moore","orcid":null,"position":2,"is_corresponding":false},{"id":628043,"name":"Nicholas T. Thomas","orcid":"0000-0003-4578-8463","position":3,"is_corresponding":false},{"id":1011286,"name":"Brooke D. Lancaster","orcid":null,"position":4,"is_corresponding":false},{"id":1011287,"name":"Kelsey A. Reeves","orcid":null,"position":5,"is_corresponding":false},{"id":628042,"name":"Alexander R. Keeble","orcid":"0000-0001-5895-4315","position":6,"is_corresponding":false},{"id":286546,"name":"Christopher S. Fry","orcid":"0000-0002-4207-6594","position":7,"is_corresponding":false},{"id":909776,"name":"Darren L. Johnson","orcid":null,"position":8,"is_corresponding":false},{"id":94862,"name":"Katherine Thompson","orcid":"0000-0002-9193-9210","position":9,"is_corresponding":false},{"id":734643,"name":"Brian Noehren","orcid":"0000-0001-9250-7439","position":10,"is_corresponding":false},{"id":628044,"name":"Jean L. Fry","orcid":"0000-0002-3066-0451","position":11,"is_corresponding":false},{"id":559626,"name":"Yuan Wen","orcid":"0000-0002-3210-1629","position":0,"is_corresponding":true}],"reference_count":69,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:11:53.085356Z","pmid":"37856482","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}