{"doi":"10.1172/jci.insight.170210","title":"Immune responses associated with protection induced by chemoattenuated PfSPZ vaccine in malaria-naive Europeans","abstract":"Vaccination of malaria-naive volunteers with a high dose of Plasmodium falciparum sporozoites chemoattenuated by chloroquine (CQ) (PfSPZ-CVac [CQ]) has previously demonstrated full protection against controlled human malaria infection (CHMI). However, lower doses of PfSPZ-CVac [CQ] resulted in incomplete protection. This provides the opportunity to understand the immune mechanisms needed for better vaccine-induced protection by comparing individuals who were protected with those not protected. Using mass cytometry, we characterized immune cell composition and responses of malaria-naive European volunteers who received either lower doses of PfSPZ-CVac [CQ], resulting in 50% protection irrespective of the dose, or a placebo vaccination, with everyone becoming infected following CHMI. Clusters of CD4+ and γδ T cells associated with protection were identified, consistent with their known role in malaria immunity. Additionally, EMRA CD8+ T cells and CD56+CD8+ T cell clusters were associated with protection. In a cohort from a malaria-endemic area in Gabon, these CD8+ T cell clusters were also associated with parasitemia control in individuals with lifelong exposure to malaria. Upon stimulation with P. falciparum-infected erythrocytes, CD4+, γδ, and EMRA CD8+ T cells produced IFN-γ and/or TNF, indicating their ability to mediate responses that eliminate malaria parasites.","journal":"JCI Insight","year":2024,"id":467936,"datarank":0.21408318562184875,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.006139031453865125,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.006139031453865125,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":3,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9543,"is_data_producer":true,"deposit_databanks":{"ClinicalTrials.gov":["NCT02115516"],"EudraCT":["2013-003900-38"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1300510,"name":"Simon P. Jochems","orcid":"0000-0002-4835-1032","position":1,"is_corresponding":false},{"id":228590,"name":"Vincent van Unen","orcid":"0000-0001-9339-8430","position":2,"is_corresponding":false},{"id":976694,"name":"Madeleine Eunice Betouke Ongwe","orcid":"0000-0002-4206-1333","position":3,"is_corresponding":false},{"id":1300795,"name":"Wouter Aa de Steenhuijsen Piters","orcid":null,"position":4,"is_corresponding":false},{"id":1300511,"name":"Koen A. Stam","orcid":"0000-0003-0982-4439","position":5,"is_corresponding":false},{"id":380313,"name":"Marguerite Massinga Loembé","orcid":"0000-0002-7822-566X","position":6,"is_corresponding":false},{"id":290873,"name":"B. Kim Lee Sim","orcid":"0000-0002-8598-8905","position":7,"is_corresponding":false},{"id":658786,"name":"Meral Esen","orcid":"0000-0002-7657-6458","position":8,"is_corresponding":false},{"id":290874,"name":"Stephen L. Hoffman","orcid":"0000-0002-0700-9505","position":9,"is_corresponding":false},{"id":313979,"name":"Peter G. Kremsner","orcid":"0000-0002-3926-4113","position":10,"is_corresponding":false},{"id":561822,"name":"Rolf Fendel","orcid":"0000-0003-4716-0311","position":11,"is_corresponding":false},{"id":380308,"name":"Benjamin Mordmüller","orcid":"0000-0001-9101-2768","position":12,"is_corresponding":false},{"id":608900,"name":"Maria Yazdanbakhsh","orcid":"0000-0002-7666-1441","position":13,"is_corresponding":false},{"id":976693,"name":"Yoanne D. Mouwenda","orcid":"0000-0002-5827-419X","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":null,"created_at":"2026-07-19T02:05:15.025255Z","pmid":"38716733","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}