{"doi":"10.1167/tvst.14.1.10","title":"Anti-Syndecan 2 Antibody Treatment Reduces Edema Formation and Inflammation of Murine Laser-Induced CNV","abstract":"Purpose: Alteration of visual acuity in wet age-related macular degeneration (AMD) is mostly driven by vascular endothelial growth factor A (VEGF-A)-induced edema from leaky newly forming blood vessels below the retina layers. To date, all therapies aimed at alleviation of this process have relied on inhibition of VEGF-A activity. Although effective in preventing vascular leak and edema, this approach also leads to the loss of normal vasculature and multiple related side effects. Methods: We have developed an alternative strategy that uses anti-syndecan-2 polyclonal antibody (anti-Sdc2 pAb) to block VEGF-A-induced permeability without interfering with other VEGF-A activities. The effect of anti-Sdc2 pAb therapy was assessed in vitro using a transendothelial electrical resistance (TEER) assay, as well as staining of the endothelial cell junction, and in vivo in the laser-induced choroidal neovascularization (CNV) model. Results: Anti-Sdc2 pAb blocked VEGF-A-induced permeability in vitro, and both local intravitreal injections and systemic intravenous treatments with anti-Sdc2 pAb were as effective as intravitreal anti-VEGF therapy in reducing edema, size of retinal lesions, and local inflammation in this model. Post-injury neovascularization was not affected by treatment with anti-Sdc2 pAb. Conclusions: These findings indicate that anti-Sdc2 pAb therapy can be an effective alternative to anti-VEGF-A approaches for suppression of edema and to prevent retinal lesions in wet neovascular AMD (nAMD). Translational Relevance: Intravitreal anti-Sdc2 treatment may avoid side effects observed with the long-term anti-VEGF therapy, and systemic treatment with an anti-Sdc2 pAb antibody can address the issues associated with repeated intravitreal injections.","journal":"Translational Vision Science & Technology","year":2025,"id":542735,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9583,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1432266,"name":"Filippo Locri","orcid":"0009-0000-3772-8730","position":1,"is_corresponding":false},{"id":1432267,"name":"Flavia Plastino","orcid":"0000-0001-8160-3273","position":2,"is_corresponding":false},{"id":334646,"name":"Paola Perrotta","orcid":"0000-0002-3551-5560","position":3,"is_corresponding":false},{"id":1432585,"name":"Krisztina M. Zsebo","orcid":null,"position":4,"is_corresponding":false},{"id":1264844,"name":"Emma Ristori","orcid":"0000-0001-9999-2780","position":5,"is_corresponding":false},{"id":1085037,"name":"Xiangyun Yin","orcid":"0000-0003-0903-2319","position":6,"is_corresponding":false},{"id":105822,"name":"Eric Song","orcid":"0000-0001-5448-5865","position":7,"is_corresponding":false},{"id":1432268,"name":"Helder André","orcid":"0000-0002-2926-2376","position":8,"is_corresponding":false},{"id":245133,"name":"Michael Simons","orcid":"0000-0003-0348-7734","position":9,"is_corresponding":false},{"id":1432265,"name":"Federico Corti","orcid":"0000-0003-2160-245X","position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":null,"created_at":"2026-07-19T02:53:00.171379Z","pmid":"39792057","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}