{"doi":"10.1167/iovs.12-11504","title":"Pretreatment With TLR2 and TLR4 Ligand Modulates Innate Immunity in Corneal Fibroblasts Challenged With<i>Aspergillus fumigatus</i>","abstract":null,"journal":"Investigative Opthalmology &amp; Visual Science","year":2013,"id":650791,"datarank":0.38474240361923057,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.0,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1696975,"name":"Hongling Yang","orcid":null,"position":1,"is_corresponding":false},{"id":1460434,"name":"Xinyi Wu","orcid":"0000-0002-4057-6675","position":2,"is_corresponding":false},{"id":1696974,"name":"Yingchao Li","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Pretreatment With TLR2 and TLR4 Ligand Modulates Innate Immunity in Corneal Fibroblasts Challenged With<i>Aspergillus fumigatus</i>","abstract":"<h4>Purpose</h4>To study the innate immunity in telomerase-immortalized human stroma fibroblasts (THSFs) challenged with Aspergillus fumigatus hyphae after copretreatment with TLR2 and TLR4 ligand.<h4>Methods</h4>THSFs were pretreated with different concentrations of zymosan and/or lipopolysaccharide (LPS) at different time periods, and challenged with high-dose Aspergillus fumigatus hyphae. The gene expression and protein secretion of inflammatory cytokines (TNF-α, IL-6, and IL-8) were detected by RT-PCR and ELISA. The effects of stimulation or pretreatment of TLR ligands on proliferation of THSFs were measured by MTT analysis.<h4>Results</h4>In the certain concentration range, pretreatment of THSFs with zymosan suppressed gene expression of inflammatory cytokines (TNF-α and IL-6). Copretreatment with zymosan and LPS suppressed gene expression and protein secretion more strongly compared with pretreatment with zymosan or LPS alone. Zymosan and/or LPS pretreatment suppressed lethal effect of A. fumigatus to THSFs in a certain period.<h4>Conclusions</h4>Pretreatment of THSFs with TLR2-specific ligand zymosan results in a state of A. fumigatus hyphae tolerance. Copretreatment with TLR2 and -4 ligands (zymosan and LPS) leads to a stronger state of A. fumigatus hyphae tolerance, and suppresses the lethal effect of A. fumigatus.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23633661","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":null,"oa_locations":[{"url":"http://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.12-11504","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["Corneal Stroma","Humans","Aspergillus fumigatus","Lipopolysaccharides","Zymosan","RNA, Messenger","Cytokines","Ligands","Enzyme-Linked Immunosorbent Assay","Drug Therapy, Combination","Cell Proliferation","Gene Expression Regulation","Toll-Like Receptor 2","Toll-Like Receptor 4","Immunity, Innate","Real-Time Polymerase Chain Reaction","Corneal Keratocytes"],"keywords":["Cytokines","Zymosan","Aspergillus fumigatus","Lipopolysaccaride","Human Stroma Fibroblasts"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T06:07:12.808567Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}